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Electromagnetic field stimulation potentiates endogenous myelin repair by recruiting subventricular neural stem cells in an experimental model of white matter demyelination.

Journal of molecular neuroscience : MN · 2012 · Benefit · Low evidence

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BenefitLow evidencePaper 4528

In a rat model of toxin-induced white matter demyelination, 60 Hz (0.7 mT) EMF exposure reduced the extent of the demyelinated area and increased MBP staining in the lesion area on days 14 and 28 postlesion. EMF exposure also increased BrdU- and nestin-positi…

Study snapshot

PublicationJournal of molecular neuroscience : MN · 2012
Study typeanimal
Reported directionBenefit
Evidence ratingLow

Abstract

Electromagnetic fields (EMFs) may affect the endogenous neural stem cells within the brain. The aim of this study was to assess the effects of EMFs on the process of toxin-induced demyelination and subsequent remyelination. Demyelination was induced using local injection of lysophosphatidylcholine within the corpus callosum of adult female Sprague-Dawley rats. EMFs (60 Hz; 0.7 mT) were applied for 2 h twice a day for 7, 14, or 28 days postlesion. BrdU labeling and immunostaining against nestin, myelin basic protein (MBP), and BrdU were used for assessing the amount of neural stem cells within the tissue, remyelination patterns, and tracing of proliferating cells, respectively. EMFs significantly reduced the extent of demyelinated area and increased the level of MBP staining within the lesion area on days 14 and 28 postlesion. EMFs also increased the number of BrdU- and nestin-positive cells within the area between SVZ and lesion as observed on days 7 and 14 postlesion. It seems that EMF potentiates proliferation and migration of neural stem cells and enhances the repair of myelin in the context of demyelinating conditions.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal
Effect directionbenefit
PopulationAdult female Sprague-Dawley rats with lysophosphatidylcholine-induced corpus callosum demyelination
Sample sizeNot supplied
ExposureELF · 2 h twice a day for 7, 14, or 28 days postlesion
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In a rat model of toxin-induced white matter demyelination, 60 Hz (0.7 mT) EMF exposure reduced the extent of the demyelinated area and increased MBP staining in the lesion area on days 14 and 28 postlesion. EMF exposure also increased BrdU- and nestin-positive cells in the area between the subventricular zone (SVZ) and the lesion on days 7 and 14 postlesion.

Outcomes measured

  • Extent of demyelinated area
  • Myelin basic protein (MBP) staining level in lesion area
  • Number of BrdU-positive cells between SVZ and lesion
  • Number of nestin-positive cells between SVZ and lesion

Limitations

  • Sample size not reported in the abstract
  • Animal model (rat) with toxin-induced demyelination; generalizability to humans not addressed in the abstract
  • Exposure parameters limited to a single frequency (60 Hz) and flux density (0.7 mT)
View raw extracted JSON
{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "2 h twice a day for 7, 14, or 28 days postlesion"
  },
  "population": "Adult female Sprague-Dawley rats with lysophosphatidylcholine-induced corpus callosum demyelination",
  "sample_size": null,
  "outcomes": [
    "Extent of demyelinated area",
    "Myelin basic protein (MBP) staining level in lesion area",
    "Number of BrdU-positive cells between SVZ and lesion",
    "Number of nestin-positive cells between SVZ and lesion"
  ],
  "main_findings": "In a rat model of toxin-induced white matter demyelination, 60 Hz (0.7 mT) EMF exposure reduced the extent of the demyelinated area and increased MBP staining in the lesion area on days 14 and 28 postlesion. EMF exposure also increased BrdU- and nestin-positive cells in the area between the subventricular zone (SVZ) and the lesion on days 7 and 14 postlesion.",
  "effect_direction": "benefit",
  "limitations": [
    "Sample size not reported in the abstract",
    "Animal model (rat) with toxin-induced demyelination; generalizability to humans not addressed in the abstract",
    "Exposure parameters limited to a single frequency (60 Hz) and flux density (0.7 mT)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic fields",
    "ELF EMF",
    "60 Hz",
    "0.7 mT",
    "demyelination",
    "remyelination",
    "myelin repair",
    "subventricular zone",
    "neural stem cells",
    "BrdU",
    "nestin",
    "myelin basic protein",
    "lysophosphatidylcholine",
    "corpus callosum",
    "Sprague-Dawley rat"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s12031-012-9791-8
PubMed: 22588976

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