[Effect of electromagnetic field of extremely low frequency on ATPase activity of actomyosin].

Ukrains'kyi biokhimichnyi zhurnal (1999 ) · 2012 · Mixed · Low evidence

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MixedLow evidencePaper 4483

Study snapshot

PublicationUkrains'kyi biokhimichnyi zhurnal (1999 ) · 2012

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

The Mg2+/Ca2+ and K(+)-ATPase actomyosin activity of rabbit skeletal muscle was evaluated by the Fiske-Subbarow method during a five-hour exposition of protein solutions in electromagnetic field of extremely low frequency of 8 Hz and 25 microT induction. The results of the study of the ATPase activity of actomyosin upon electromagnetic exposure have shown statistically significant changes that are characterized by a rather complex time dynamics. After 1, 2 and 4 hours of exposure of protein solutions the effect of ELF EMF exposure inhibits the ATPase activity compared to control samples, which are not exposed to the magnetic field. By the third and fifth hours of exposure to the electromagnetic field, there is a significant increase in the ATPase activity of actomyosin. It should be noted that a similar pattern of change in enzyme activity was universal, both for the environment by Mg2+ and Ca2+, and in the absence of these ions in the buffer. This can evidence for Ca(2+)-independent ways of the infuence of electromagnetic field (EMP) on biologic objects. In our opinion, the above effects are explained by EMP influence on the dynamic properties of actomyosin solutions, which are based on the processes of spontaneous dynamic formation of structure.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationRabbit skeletal muscle actomyosin protein solutions (in vitro)

Sample sizeNot supplied

ExposureELF · 8e-06 MHz · 5 hours

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

During 5-hour exposure of rabbit skeletal muscle actomyosin protein solutions to an 8 Hz, 25 microT extremely low frequency electromagnetic field, ATPase activity showed statistically significant time-dependent changes. Activity was inhibited versus control after 1, 2, and 4 hours, and increased after 3 and 5 hours; a similar pattern was observed with Mg2+/Ca2+ present and in their absence.

Outcomes measured

Mg2+/Ca2+-ATPase activity of actomyosin
K(+)-ATPase activity of actomyosin

Limitations

In vitro protein-solution experiment; findings may not generalize to intact organisms or humans
Sample size not reported in the abstract
Only one frequency (8 Hz) and one magnetic induction level (25 microT) described
Exposure duration limited to 5 hours; longer-term effects not assessed

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": 8e-06,
    "sar_wkg": null,
    "duration": "5 hours"
  },
  "population": "Rabbit skeletal muscle actomyosin protein solutions (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Mg2+/Ca2+-ATPase activity of actomyosin",
    "K(+)-ATPase activity of actomyosin"
  ],
  "main_findings": "During 5-hour exposure of rabbit skeletal muscle actomyosin protein solutions to an 8 Hz, 25 microT extremely low frequency electromagnetic field, ATPase activity showed statistically significant time-dependent changes. Activity was inhibited versus control after 1, 2, and 4 hours, and increased after 3 and 5 hours; a similar pattern was observed with Mg2+/Ca2+ present and in their absence.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro protein-solution experiment; findings may not generalize to intact organisms or humans",
    "Sample size not reported in the abstract",
    "Only one frequency (8 Hz) and one magnetic induction level (25 microT) described",
    "Exposure duration limited to 5 hours; longer-term effects not assessed"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "ELF EMF",
    "8 Hz",
    "25 microT",
    "actomyosin",
    "ATPase activity",
    "rabbit skeletal muscle",
    "Fiske-Subbarow method",
    "time dynamics",
    "enzyme activity"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 23342636

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