STAT3 signalling pathway is involved in the activation of microglia induced by 2.45 GHz electromagnetic fields.

International journal of radiation biology · 2010 · Mixed · Low evidence

← Research stream

MixedLow evidencePaper 4400

Exposure to 2.45 GHz EMF increased STAT3 DNA-binding ability, phosphorylation of STAT3, activation of JAK1 and JAK2, and transcription of inflammation-associated genes iNOS and TNF-alpha in murine microglial cells. A JAK inhibitor reduced these effects, indic…

Study snapshot

PublicationInternational journal of radiation biology · 2010

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

PURPOSE: Microglia activation plays a pivotal role in the initiation and progression of central nervous system (CNS) insult. The aim of the present work was to investigate the activation of microglia and involvement of signal transducer and activator of transcription 3 (STAT3) in microglia activation after 2.45 GHz electromagnetic fields (EMF) exposure. MATERIALS AND METHODS: In this study, murine N9 microglial cells were exposed to 2.45 GHz EMF, the protein expressions of STAT3, Janus Tyrosine kinase 1 and 2(JAK1 and JAK2), phosphor-(Try705)STAT3 and DNA binding activity of STAT3 were examined by Western blot analysis and electrophoresis mobility shift assay (EMSA). Levels of the nitric oxide (NO) derivative nitrite were determined in the culture medium by the Griess reaction. The mRNA expression of tumour necrosis factor alpha (TNF-alpha) and inducible nitric oxide synthase (iNOS) were detected by reverse transcription and polymerase chain reaction (RT-PCR). RESULTS: A significant increase of STAT3 DNA-binding ability was noted after exposure. Consistent with this, EMF rapidly induced phosphorylation of STAT3 and activated JAK1 and JAK2. In addition, EMF exposure increased transcription levels of the inflammation-associated genes, iNOS and TNF-alpha, which are reported to contain STAT-binding elements in their promoter region. P6, a JAK inhibitor, reduced induction of iNOS and TNF-alpha, nuclear factor binding activity, and activation of STAT3 in EMF-stimulated microglia. CONCLUSION: These results provide evidence that EMF exposure can initiate the activation of microglia cells and STAT3 signalling involves in EMF-induced microglial activation.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

Populationmurine N9 microglial cells

Sample sizeNot supplied

Exposuremicrowave · 2450 MHz

Evidence strengthLowConfidence: 30% · Peer reviewed: yes

Main findings

Outcomes measured

STAT3 DNA-binding ability
phosphorylation of STAT3
activation of JAK1 and JAK2
transcription levels of iNOS and TNF-alpha
nitric oxide derivative nitrite levels

Limitations

In vitro study using murine microglial cell line, limiting direct applicability to humans
No information on exposure duration or dose-response
No assessment of functional or behavioral outcomes

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": 2450,
    "sar_wkg": null,
    "duration": null
  },
  "population": "murine N9 microglial cells",
  "sample_size": null,
  "outcomes": [
    "STAT3 DNA-binding ability",
    "phosphorylation of STAT3",
    "activation of JAK1 and JAK2",
    "transcription levels of iNOS and TNF-alpha",
    "nitric oxide derivative nitrite levels"
  ],
  "main_findings": "Exposure to 2.45 GHz EMF increased STAT3 DNA-binding ability, phosphorylation of STAT3, activation of JAK1 and JAK2, and transcription of inflammation-associated genes iNOS and TNF-alpha in murine microglial cells. A JAK inhibitor reduced these effects, indicating involvement of STAT3 signaling in microglial activation induced by EMF.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study using murine microglial cell line, limiting direct applicability to humans",
    "No information on exposure duration or dose-response",
    "No assessment of functional or behavioral outcomes"
  ],
  "evidence_strength": "low",
  "confidence": 0.3,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "STAT3",
    "microglia",
    "2.45 GHz",
    "electromagnetic fields",
    "JAK1",
    "JAK2",
    "iNOS",
    "TNF-alpha",
    "inflammation",
    "signal transduction"
  ],
  "suggested_hubs": []
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3109/09553000903264507
PubMed: 20070213

Read the original paper →

Related papers

← Back to the Research Library