Biochemical and structural approach to collagen synthesis under electric fields.

General physiology and biophysics · 1996 · Mixed · Low evidence

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MixedLow evidencePaper 4195

In guinea pigs, 0.9 kV/m vertical and horizontal DC electric fields decreased liver hydroxyproline content, while 1.9 kV/m fields increased hydroxyproline content. Vertical fields were reported as more effective than horizontal fields for both increases and d…

Study snapshot

PublicationGeneral physiology and biophysics · 1996

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

The effects of electric fields of different intensities and directions on the collagen synthesis of guinea pigs was studied. The effects of vertical and horizontal electric fields of 1.9 kV/m and 0.9 kV/m, generated with voltages of 300 V and 150 V DC, respectively, on collagen synthesis, was evaluated by assessing the amount of hydroxyproline in the liver tissue, and by histological examination of tissue samples. The Stegemann-Stalder method was used to determine hydroxyproline content of the tissues. While the 0.9 kV/m vertical and horizontal electric fields substantially decreased the amount of hydroxyproline in the liver, the 1.9 kV/m electric fields in both directions increased the hydroxyproline content. The vertical electric field was more effective than the horizontal one both in the increases and the decreases. These findings were verified histologically.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionmixed

Populationguinea pigs

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Liver tissue hydroxyproline content (collagen synthesis proxy)
Histological tissue changes related to collagen synthesis

Limitations

Sample size not reported in abstract
Exposure duration not reported in abstract
Limited outcome scope (hydroxyproline in liver and histology only)
Details of experimental design (randomization/blinding/control conditions) not described in abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": null,
    "source": "electric field (DC)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "guinea pigs",
  "sample_size": null,
  "outcomes": [
    "Liver tissue hydroxyproline content (collagen synthesis proxy)",
    "Histological tissue changes related to collagen synthesis"
  ],
  "main_findings": "In guinea pigs, 0.9 kV/m vertical and horizontal DC electric fields decreased liver hydroxyproline content, while 1.9 kV/m fields increased hydroxyproline content. Vertical fields were reported as more effective than horizontal fields for both increases and decreases, and findings were verified histologically.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure duration not reported in abstract",
    "Limited outcome scope (hydroxyproline in liver and histology only)",
    "Details of experimental design (randomization/blinding/control conditions) not described in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "DC electric field",
    "electric field intensity",
    "field direction",
    "collagen synthesis",
    "hydroxyproline",
    "liver",
    "guinea pig",
    "histology"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 9248829

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