RF Safe

[Effects of low frequency pulsed magnetic field on the proliferation and differentiation of HepG2 cells].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases · 2003 · No effect · Low evidence

← Research stream
No effectLow evidencePaper 3732

No significant differences in cell proliferation were observed between sham and exposed groups at 80 Hz, 1.55 mT for 1–24 h. No significant differences in cell proliferation or AFP secretion were observed between sham and exposed groups at 16 Hz, 1.55 mT for …

Study snapshot

PublicationZhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases · 2003
Study typein_vitro
Reported directionNo effect
Evidence ratingLow

Abstract

OBJECTIVE: To study the effects of low frequency pulsed magnetic field on the proliferation and differentiation of HepG2 cells. METHODS: 3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium (MTS) colorimetry method and ELISA assay of alpha-fetoprotein (AFP) were used to determine the cell proliferation and differentiation after the cells were exposed to pulsed magnetic fields with different frequency but the same field intensity. RESULTS: There were no significant differences in cell proliferation between sham and treated groups exposed to the field of 80 Hz, 1.55 mT for 1, 4, 8, 12, 24 h (P > 0.05). There were also no significant differences in cell proliferation and AFP secretion between sham and treated groups exposed to 16 Hz, 1.55 mT pulsed magnetic fields for 1, 4, 8, 24 h (P > 0.05). CONCLUSION: There were no "window effects" found in HepG2 cells proliferation or AFP secretion at 16 Hz and 80 Hz pulsed magnetic fields.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionno_effect
PopulationHepG2 cells
Sample sizeNot supplied
ExposureELF · 1, 4, 8, 12, 24 h (80 Hz); 1, 4, 8, 24 h (16 Hz)
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

No significant differences in cell proliferation were observed between sham and exposed groups at 80 Hz, 1.55 mT for 1–24 h. No significant differences in cell proliferation or AFP secretion were observed between sham and exposed groups at 16 Hz, 1.55 mT for 1–24 h; no “window effects” were found at 16 Hz or 80 Hz.

Outcomes measured

  • Cell proliferation (MTS assay)
  • Alpha-fetoprotein (AFP) secretion (ELISA)
  • Cell differentiation (via AFP secretion)

Limitations

  • In vitro study (HepG2 cell line); generalizability to humans not addressed
  • Sample size not reported in abstract
  • Only two frequencies tested at a single field intensity (1.55 mT)
  • Limited endpoints reported (proliferation and AFP secretion)
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "pulsed magnetic field",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "1, 4, 8, 12, 24 h (80 Hz); 1, 4, 8, 24 h (16 Hz)"
  },
  "population": "HepG2 cells",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation (MTS assay)",
    "Alpha-fetoprotein (AFP) secretion (ELISA)",
    "Cell differentiation (via AFP secretion)"
  ],
  "main_findings": "No significant differences in cell proliferation were observed between sham and exposed groups at 80 Hz, 1.55 mT for 1–24 h. No significant differences in cell proliferation or AFP secretion were observed between sham and exposed groups at 16 Hz, 1.55 mT for 1–24 h; no “window effects” were found at 16 Hz or 80 Hz.",
  "effect_direction": "no_effect",
  "limitations": [
    "In vitro study (HepG2 cell line); generalizability to humans not addressed",
    "Sample size not reported in abstract",
    "Only two frequencies tested at a single field intensity (1.55 mT)",
    "Limited endpoints reported (proliferation and AFP secretion)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF",
    "pulsed magnetic field",
    "HepG2",
    "cell proliferation",
    "alpha-fetoprotein",
    "AFP",
    "MTS",
    "ELISA",
    "window effect",
    "16 Hz",
    "80 Hz",
    "1.55 mT"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.35,
      "reason": "Published in an industrial hygiene/occupational diseases journal and studies low-frequency magnetic field exposure."
    }
  ]
}
AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 14761390

Read the original paper →

Related papers

← Back to the Research Library