[Effect of extremely low frequency magnetic fields on intracellular free calcium in HepG2 cells].

Zhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases · 2003 · No effect · Low evidence

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No effectLow evidencePaper 3731

No statistically significant differences in Fura-2 fluorescence ratio measures were observed between control and exposed HepG2 cells under 1.55 mT 16 Hz pulsed exposure, 300 mT 2 Hz rotating exposure, or during real-time 0.9 mT 16 Hz sinusoidal exposure (P > …

Study snapshot

PublicationZhonghua lao dong wei sheng zhi ye bing za zhi = Zhonghua laodong weisheng zhiyebing zazhi = Chinese journal of industrial hygiene and occupational diseases · 2003

Study typein_vitro

Reported directionNo effect

Evidence ratingLow

Abstract

OBJECTIVE: To study the effect of extremely low frequency magnetic fields on intracellular calcium concentration ([Ca(2+)]i). METHODS: Fura-2 loaded HepG2 cells were exposed to 1.55 mT (average value), 16 Hz pulsed magnetic fields for 60 min and to 300 mT, 2 Hz rotating magnetic fields for 5 min, and then [Ca(2+)]i was measured by fluorescence spectrophotometer. [Ca(2+)]i of HepG2 cells was also measured when they were exposed to 0.9 mT [root mean square (rms)], 16 Hz sinusoidal magnetic fields in real time. RESULTS: The R values (F(340) nm/F(380) nm) of the control and the exposed group were 2.4519 +/- 0.2378 and 2.5266 +/- 0.2915 respectively after HepG2 cells were exposed to 1.55 mT, 16 Hz magnetic fields, 1.365 0 +/- 0.0626 and 1.3602 +/- 0.0771 respectively to 300 mT, 2 Hz rotating magnetic fields. The ratios of the trendline slope [r((501 - 1,000)) / r((0 - 500))] from the data of R values were 1.1213 +/- 0.4559 and 1.0727 +/- 0.1971 respectively (P > 0.05), and the ratios of the intercept [b((501 - 1,000)) / b((0 - 500))] from the trendline were 0.9912 +/- 0.0098 and 0.9979 +/- 0.0060 (P > 0.05) when HepG2 cells were exposed to the 0.9 mT, 16 Hz sinusoidal magnetic fields. CONCLUSION: The effect of extremely low frequency magnetic fields on [Ca(2+)]i of HepG2 cells under the experimental condition has not been found.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionno_effect

PopulationHepG2 cells

Sample sizeNot supplied

ExposureELF · 60 min (1.55 mT, 16 Hz pulsed); 5 min (300 mT, 2 Hz rotating); real-time measurement during 0.9 mT, 16 Hz sinusoidal exposure

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

intracellular free calcium concentration ([Ca2+]i)
Fura-2 fluorescence ratio R (F340/F380)

Limitations

Sample size not reported in abstract
Only one cell line (HepG2) studied
Limited exposure conditions/durations reported (specific field types and short durations)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "60 min (1.55 mT, 16 Hz pulsed); 5 min (300 mT, 2 Hz rotating); real-time measurement during 0.9 mT, 16 Hz sinusoidal exposure"
  },
  "population": "HepG2 cells",
  "sample_size": null,
  "outcomes": [
    "intracellular free calcium concentration ([Ca2+]i)",
    "Fura-2 fluorescence ratio R (F340/F380)"
  ],
  "main_findings": "No statistically significant differences in Fura-2 fluorescence ratio measures were observed between control and exposed HepG2 cells under 1.55 mT 16 Hz pulsed exposure, 300 mT 2 Hz rotating exposure, or during real-time 0.9 mT 16 Hz sinusoidal exposure (P > 0.05). The authors conclude no effect on intracellular free calcium under the experimental conditions.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in abstract",
    "Only one cell line (HepG2) studied",
    "Limited exposure conditions/durations reported (specific field types and short durations)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low frequency",
    "magnetic fields",
    "ELF",
    "HepG2",
    "intracellular calcium",
    "Fura-2",
    "fluorescence spectrophotometer",
    "pulsed magnetic field",
    "rotating magnetic field",
    "sinusoidal magnetic field"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.35,
      "reason": "Published in an industrial hygiene/occupational diseases journal and studies ELF magnetic field exposure effects (though in vitro)."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 14761392

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