Quantitative analysis of adherent cell orientation influenced by strong magnetic fields.

IEEE transactions on nanobioscience · 2003 · Mixed · Low evidence

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MixedLow evidencePaper 3697

Study snapshot

PublicationIEEE transactions on nanobioscience · 2003

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

This paper examines the effect of strong magnetic fields on adherent cells. Smooth muscle cells, cultured in 8- or 14-T superconducting magnets for three days, exhibited orientational order parallel to the magnetic field direction. To discuss the process and the mechanism of the orientation, the orientational characteristic of the cell culture was investigated with quantitative measurements: an orientational order parameter and the Fourier transform (FT) analysis. The orientational order parameter indicates the degree of orientation. The value of the parameter was estimated with the FT of microscopic images. The cells cultured under stronger static magnetic fields exhibited stronger ordering, while they showed weaker ordering in the control when they were cultured under a strong magnetic gradient force of 400 T2/m. The ordering was enhanced under uniform strong magnetic fields, while it was not affected or was suppressed by the strong gradient force. We suggest that the cells organize themselves to minimize their diamagnetic torsion stresses, which can be induced in the uniform magnetic fields by the membrane's diamagnetic anisotropy.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationCultured smooth muscle cells (adherent cells)

Sample sizeNot supplied

Exposure3 days

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Smooth muscle cells cultured for three days in 8 T or 14 T superconducting magnets exhibited orientational order parallel to the magnetic field direction. Stronger uniform static magnetic fields produced stronger ordering, whereas in a control condition with a strong magnetic gradient force (400 T^2/m) ordering was weaker; the gradient force condition was reported as not affecting or suppressing ordering.

Outcomes measured

Cell orientation/orientational order (order parameter)
Fourier transform (FT) analysis of microscopic images

Limitations

Sample size not reported in abstract
In vitro cell culture study; generalizability to in vivo/humans not addressed
Exposure characterization limited to field strengths and a single gradient force value; other parameters not described

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": "superconducting magnets (static magnetic fields)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "3 days"
  },
  "population": "Cultured smooth muscle cells (adherent cells)",
  "sample_size": null,
  "outcomes": [
    "Cell orientation/orientational order (order parameter)",
    "Fourier transform (FT) analysis of microscopic images"
  ],
  "main_findings": "Smooth muscle cells cultured for three days in 8 T or 14 T superconducting magnets exhibited orientational order parallel to the magnetic field direction. Stronger uniform static magnetic fields produced stronger ordering, whereas in a control condition with a strong magnetic gradient force (400 T^2/m) ordering was weaker; the gradient force condition was reported as not affecting or suppressing ordering.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "In vitro cell culture study; generalizability to in vivo/humans not addressed",
    "Exposure characterization limited to field strengths and a single gradient force value; other parameters not described"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "static magnetic field",
    "superconducting magnet",
    "8 T",
    "14 T",
    "magnetic gradient force",
    "400 T2/m",
    "smooth muscle cells",
    "cell orientation",
    "diamagnetic anisotropy",
    "Fourier transform",
    "orientational order parameter"
  ],
  "suggested_hubs": [
    {
      "slug": "occupational-exposure",
      "weight": 0.2,
      "reason": "Study involves very strong static magnetic fields (8–14 T) relevant to high-field magnet environments, though no occupational context is explicitly stated."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1109/tnb.2003.810157
PubMed: 15382419

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