Impact of weak radiofrequency and static magnetic fields on key signaling molecules, intracellular pH, membrane potential, and cell growth in HT-1080 fibrosarcoma cells

2023 · Mixed · Low evidence

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MixedLow evidencePaper 406

Study snapshot

Publicationmanual · 2023

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Impact of weak radiofrequency and static magnetic fields on key signaling molecules, intracellular pH, membrane potential, and cell growth in HT-1080 fibrosarcoma cells Gurhan H, Barnes F. Impact of weak radiofrequency and static magnetic fields on key signaling molecules, intracellular pH, membrane potential, and cell growth in HT-1080 fibrosarcoma cells. Sci Rep. 2023;13(1):14223. Published 2023 Aug 30. doi:10.1038/s41598-023-41167-5 Abstract There are substantial concerns that extended exposures to weak radiofrequency (RF) fields can lead to adverse health effects. In this study, HT-1080 fibrosarcoma cells were simultaneously exposed to a static magnetic flux density between 10 μT and 300 μT and RF magnetic fields with amplitudes ranging from 1 nT to 1.5 μT in the frequency range from 1.8 to 7.2 MHz for four days. Cell growth rates, intracellular pH, hydrogen peroxide, peroxynitrite, membrane potential and mitochondrial calcium were measured. Results were dependent on carrier frequency and the magnitude of the RF magnetic field, modulation frequencies and the background static magnetic field (SMF). Iron sulphur (Fe-S) clusters are essential for the generation of reactive oxygen species and reactive nitrogen species (ROS and RNS). We believe the observed changes are associated with hyperfine couplings between the chemically active electrons and nuclear spins. Controlling external magnetic fields may have important clinical implications on aging, cancer, arthritis, and Alzheimer’s. Open access paper: nature.com

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHT-1080 fibrosarcoma cells

Sample sizeNot supplied

ExposureRF · four days

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

HT-1080 fibrosarcoma cells were simultaneously exposed to static magnetic fields (10–300 µT) and RF magnetic fields (1 nT–1.5 µT; 1.8–7.2 MHz) for four days. Measured outcomes (cell growth, intracellular pH, hydrogen peroxide, peroxynitrite, membrane potential, mitochondrial calcium) showed results that depended on carrier frequency, RF field magnitude, modulation frequencies, and background static magnetic field.

Outcomes measured

cell growth rates
intracellular pH
hydrogen peroxide
peroxynitrite
membrane potential
mitochondrial calcium

Limitations

In vitro cell model; findings may not generalize to humans or in vivo conditions
Sample size not reported in the provided abstract
Direction and magnitude of changes for each endpoint are not specified in the abstract
Exposure metrics reported as magnetic field amplitudes/flux density; SAR not provided

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "four days"
  },
  "population": "HT-1080 fibrosarcoma cells",
  "sample_size": null,
  "outcomes": [
    "cell growth rates",
    "intracellular pH",
    "hydrogen peroxide",
    "peroxynitrite",
    "membrane potential",
    "mitochondrial calcium"
  ],
  "main_findings": "HT-1080 fibrosarcoma cells were simultaneously exposed to static magnetic fields (10–300 µT) and RF magnetic fields (1 nT–1.5 µT; 1.8–7.2 MHz) for four days. Measured outcomes (cell growth, intracellular pH, hydrogen peroxide, peroxynitrite, membrane potential, mitochondrial calcium) showed results that depended on carrier frequency, RF field magnitude, modulation frequencies, and background static magnetic field.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro cell model; findings may not generalize to humans or in vivo conditions",
    "Sample size not reported in the provided abstract",
    "Direction and magnitude of changes for each endpoint are not specified in the abstract",
    "Exposure metrics reported as magnetic field amplitudes/flux density; SAR not provided"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radiofrequency",
    "static magnetic field",
    "HT-1080",
    "fibrosarcoma",
    "intracellular pH",
    "membrane potential",
    "hydrogen peroxide",
    "peroxynitrite",
    "mitochondrial calcium",
    "cell growth",
    "Fe-S clusters",
    "ROS",
    "RNS",
    "hyperfine coupling"
  ],
  "suggested_hubs": [
    {
      "slug": "mechanisms-ros-rns",
      "weight": 0.78,
      "reason": "Measures ROS/RNS-related endpoints (hydrogen peroxide, peroxynitrite) and discusses Fe-S clusters/hyperfine coupling mechanisms."
    },
    {
      "slug": "static-magnetic-fields",
      "weight": 0.7,
      "reason": "Includes simultaneous exposure to background static magnetic flux density (10–300 µT)."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1038/s41598-023-41167-5
PubMed: Not supplied

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