[DNA damage and repair induced by acute exposure of microwave from mobile phone on cultured human lens epithelial cells].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology · 2006 · Mixed · Low evidence

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MixedLow evidencePaper 3493

Study snapshot

Publication[Zhonghua yan ke za zhi] Chinese journal of ophthalmology · 2006

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

OBJECTIVE: To investigate the effects of acute exposure of low-power 217 Hz modulated 1. 8 GHz microwave radiation on the DNA damage of human lens epithelial cells (hLECs) and repair. METHODS: Cultured hLECs were exposed to 217 Hz modulated 1. 8 GHz microwave radiation at SAR (specific absorption rate) of 1. 0, 2. 0, 3. O0 and 4. 0 W/kg for 2 hours in an sXc-1800 incubator and irradiate system, the DNA single strand breaks were detected with comet assay ( single-cell gel electrophoresis) in sham-irradiated cells and irradiated cells incubated for varying periods: 0, 30 and 60 minutes after irradiation. Images of comets were digitized and analyzed using an Imagine-pro plus software, and the indexes used in this study were tail length (TL) and tail moment (TM). BrdU was added into the medium with additional one hour incubation after radiation, the cell proliferation rate was determined using a BrdU-kit. RESULTS: The difference of DNA-breaks between the exposure and sham exposure groups induced by 1.0 and 2.0 W/kg irradiation were not significant in each time points (P > 0.05) ; there were significant difference in both groups at the exposure dose of 3. 0 and 4. 0 W/kg immediately and at the time of 30 minutes after irradiation (P <0. 01) ; if the radiation exposure time was beyond one hour no differences were be able to detected in 3.0 W/kg group (P > 0. 05) compared with control, but the evidence of significant DNA damage still existed in 4. 0 W/kg group at the same time point. Cell proliferation rate had no significant difference when the application of SAR was < or = 3. 0 W/kg (P >0. 05) , however the cell proliferation was decreased significantly at the dose of 4. 0 W/kg irradiation ( P < 0. 01). CONCLUSIONS: No effective DNA damage was induced using comet assay after 2 hours irradiation of 1. 8 GHz microwave on hLECs at the dose SAR < or = 3.0 W/kg. 4.0 W/kg irradiation caused significantly DNA damage and inhibition of hLECs proliferation.

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Study typein_vitro

Effect directionmixed

PopulationCultured human lens epithelial cells (hLECs)

Sample sizeNot supplied

ExposureRF · 1800 MHz · 4 W/kg · 2 hours

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Cultured human lens epithelial cells exposed for 2 hours to 217 Hz-modulated 1.8 GHz microwave radiation showed no significant DNA single-strand breaks at SAR 1.0 or 2.0 W/kg at any assessed time point. Significant DNA damage was observed at SAR 3.0 and 4.0 W/kg immediately and 30 minutes post-exposure; at 60 minutes post-exposure, no difference was detected at 3.0 W/kg but significant DNA damage persisted at 4.0 W/kg. Cell proliferation was not significantly changed at SAR ≤3.0 W/kg but was significantly decreased at 4.0 W/kg.

Outcomes measured

DNA single strand breaks (comet assay; tail length, tail moment)
Cell proliferation rate (BrdU assay)

Limitations

Sample size not reported in abstract
In vitro cell culture model; generalizability to humans not established in abstract
Acute exposure only (2 hours)
DNA damage assessed only by comet assay endpoints (tail length, tail moment)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": "mobile phone",
    "frequency_mhz": 1800,
    "sar_wkg": 4,
    "duration": "2 hours"
  },
  "population": "Cultured human lens epithelial cells (hLECs)",
  "sample_size": null,
  "outcomes": [
    "DNA single strand breaks (comet assay; tail length, tail moment)",
    "Cell proliferation rate (BrdU assay)"
  ],
  "main_findings": "Cultured human lens epithelial cells exposed for 2 hours to 217 Hz-modulated 1.8 GHz microwave radiation showed no significant DNA single-strand breaks at SAR 1.0 or 2.0 W/kg at any assessed time point. Significant DNA damage was observed at SAR 3.0 and 4.0 W/kg immediately and 30 minutes post-exposure; at 60 minutes post-exposure, no difference was detected at 3.0 W/kg but significant DNA damage persisted at 4.0 W/kg. Cell proliferation was not significantly changed at SAR ≤3.0 W/kg but was significantly decreased at 4.0 W/kg.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in abstract",
    "In vitro cell culture model; generalizability to humans not established in abstract",
    "Acute exposure only (2 hours)",
    "DNA damage assessed only by comet assay endpoints (tail length, tail moment)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "1.8 GHz",
    "217 Hz modulation",
    "microwave radiation",
    "radiofrequency",
    "specific absorption rate",
    "SAR",
    "human lens epithelial cells",
    "comet assay",
    "DNA single strand breaks",
    "BrdU",
    "cell proliferation"
  ],
  "suggested_hubs": [
    {
      "slug": "mobile-phones-rf",
      "weight": 0.9,
      "reason": "Exposure described as 1.8 GHz microwave from mobile phone with SAR values."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 17415965

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