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Nanosecond pulse electric field activation of platelet-rich plasma reduces myocardial infarct size and improves left ventricular mechanical function in the rabbit heart.

The journal of extra-corporeal technology · 2012 · Benefit · Low evidence

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BenefitLow evidencePaper 3158

In a rabbit ischemia/reperfusion model, hearts treated with PRP (prepared using nsPEF) had reduced infarct size and faster left ventricular contraction and relaxation compared with saline. In H9c2 cells, nsPEF-activated PRP reduced hydrogen peroxide–stimulate…

Study snapshot

PublicationThe journal of extra-corporeal technology · 2012
Study typeanimal
Reported directionBenefit
Evidence ratingLow

Abstract

In the current study, we used the novel, nonchemical method of nanosecond pulsed electric fields (nsPEF) to investigate the efficiency of a protocol involving the in vivo treatment of the ischemic and reperfused heart and heart cells in culture with platelet-rich plasma (PRP). Associated with the restoration of blood flow to the ischemic tissue is a phenomenon referred to as "ischemic reperfusion injury." Clinically a type of reperfusion injury occurs during coronary bypass surgery once blood perfusion to the heart is restarted. Although the restoration of oxygen to ischemic myocardial cells is critical for tissue survival, reperfusion causes myocardial oxidative stress, attributable in part to the increased production of reactive oxygen species (ROS). Enhanced ROS production is associated with mitochondrial damage. Adult female New Zealand white rabbits were anesthetized and a left thoracotomy performed to expose the heart. The distal segment of the left anterior descending coronary artery was occluded for 15 minutes and then released so reperfusion of the tissue could occur. PRP (.21 mg/heart) or saline was injected into the ischemic area of the myocardium. Mechanical function of the left ventricle was analyzed using a Millar catheter attached to a Micro-Med Analysis System. H9c2 cells in culture were treated with 1 mL of nsPEF activated PRP (1.05 mg/flask) for 24 hours before analysis for ROS production or mitochondrial depolarization damage). The left ventricle contracted and relaxed faster and infarct size was reduced in hearts treated with PRP compared with saline. ROS production and mitochondrial depolarization were reduced in H9c2 cells treated with PRP and stimulated with hydrogen peroxide. These results provide evidence that nsPEFs can successfully be used to prepare PRP and that the PRP is functional in heart protection possibly by reducing ROS generation and stabilizing the mitochondria of the ischemic/reperfused heart.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal
Effect directionbenefit
PopulationAdult female New Zealand white rabbits; H9c2 cells in culture
Sample sizeNot supplied
ExposureNot supplied
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In a rabbit ischemia/reperfusion model, hearts treated with PRP (prepared using nsPEF) had reduced infarct size and faster left ventricular contraction and relaxation compared with saline. In H9c2 cells, nsPEF-activated PRP reduced hydrogen peroxide–stimulated ROS production and mitochondrial depolarization.

Outcomes measured

  • Myocardial infarct size
  • Left ventricular mechanical function (contraction/relaxation)
  • Reactive oxygen species (ROS) production in H9c2 cells
  • Mitochondrial depolarization damage in H9c2 cells

Limitations

  • Sample size not reported in the abstract
  • nsPEF exposure parameters (e.g., field strength, pulse characteristics) not reported in the abstract
  • Animal model and cell culture findings may not generalize to humans
View raw extracted JSON
{
  "study_type": "animal",
  "exposure": {
    "band": null,
    "source": "nanosecond pulsed electric fields (nsPEF) used to activate platelet-rich plasma (PRP)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Adult female New Zealand white rabbits; H9c2 cells in culture",
  "sample_size": null,
  "outcomes": [
    "Myocardial infarct size",
    "Left ventricular mechanical function (contraction/relaxation)",
    "Reactive oxygen species (ROS) production in H9c2 cells",
    "Mitochondrial depolarization damage in H9c2 cells"
  ],
  "main_findings": "In a rabbit ischemia/reperfusion model, hearts treated with PRP (prepared using nsPEF) had reduced infarct size and faster left ventricular contraction and relaxation compared with saline. In H9c2 cells, nsPEF-activated PRP reduced hydrogen peroxide–stimulated ROS production and mitochondrial depolarization.",
  "effect_direction": "benefit",
  "limitations": [
    "Sample size not reported in the abstract",
    "nsPEF exposure parameters (e.g., field strength, pulse characteristics) not reported in the abstract",
    "Animal model and cell culture findings may not generalize to humans"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "nanosecond pulsed electric fields",
    "nsPEF",
    "platelet-rich plasma",
    "PRP",
    "ischemia-reperfusion injury",
    "myocardial infarct size",
    "left ventricular function",
    "reactive oxygen species",
    "mitochondria",
    "rabbit",
    "H9c2 cells"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 23441560

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