Dielectric aggregation kinetics of cells in a uniform AC electric field.

Biorheology · 2014 · Unclear · Insufficient evidence

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UnclearInsufficient evidencePaper 3039

Study snapshot

PublicationBiorheology · 2014

Study typeother

Reported directionUnclear

Evidence ratingInsufficient

Abstract

BACKGROUND: Cell manipulation and separation technologies have potential biological and medical applications, including advanced clinical protocols such as tissue engineering. OBJECTIVE: An aggregation model was developed for a human carcinoma (HeLa) cell suspension exposed to a uniform AC electric field, in order to explore the field-induced structure formation and kinetics of cell aggregates. METHODS: The momentum equations of cells under the action of the dipole-dipole interaction were solved theoretically and the total time required to form linear string-like cluster was derived. The results were compared with those of a numerical simulation. Experiments using HeLa cells were also performed for comparison. RESULTS: The total time required to form linear string-like clusters was derived from a simple theoretical model of the cell cluster kinetics. The growth rates of the average string length of cell aggregates showed good agreement with those of the numerical simulation. In the experiment, cells were found to form massive clusters on the bottom of a chamber. The results imply that the string-like cluster grows rapidly by longitudinal attraction when the electric field is first applied and that this process slows at later times and is replaced by lateral coagulation of short strings. CONCLUSIONS: The findings presented here are expected to enable design of methods for the organization of three-dimensional (3D) cellular structures without the use of micro-fabricated substrates, such as 3D biopolymer scaffolds, to manipulate cells into spatial arrangement.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeother

Effect directionunclear

PopulationHeLa (human carcinoma) cell suspension

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthInsufficientConfidence: 74% · Peer reviewed: yes

Main findings

A theoretical model derived the total time required to form linear string-like cell clusters and showed growth rates of average string length in good agreement with numerical simulation. Experiments found cells formed massive clusters on the bottom of a chamber; results suggest rapid initial longitudinal attraction followed by slower growth and lateral coagulation at later times.

Outcomes measured

Field-induced cell aggregation/structure formation
Kinetics of cell aggregate (string-like cluster) formation
Growth rate of average string length
Formation of massive clusters in chamber experiment

Limitations

Frequency and field strength parameters not reported in the abstract
Sample size and experimental details not reported in the abstract
Primarily modeling/simulation with limited experimental description in abstract

View raw extracted JSON

{
  "study_type": "other",
  "exposure": {
    "band": null,
    "source": "uniform AC electric field",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "HeLa (human carcinoma) cell suspension",
  "sample_size": null,
  "outcomes": [
    "Field-induced cell aggregation/structure formation",
    "Kinetics of cell aggregate (string-like cluster) formation",
    "Growth rate of average string length",
    "Formation of massive clusters in chamber experiment"
  ],
  "main_findings": "A theoretical model derived the total time required to form linear string-like cell clusters and showed growth rates of average string length in good agreement with numerical simulation. Experiments found cells formed massive clusters on the bottom of a chamber; results suggest rapid initial longitudinal attraction followed by slower growth and lateral coagulation at later times.",
  "effect_direction": "unclear",
  "limitations": [
    "Frequency and field strength parameters not reported in the abstract",
    "Sample size and experimental details not reported in the abstract",
    "Primarily modeling/simulation with limited experimental description in abstract"
  ],
  "evidence_strength": "insufficient",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "AC electric field",
    "dielectric aggregation",
    "dipole-dipole interaction",
    "cell manipulation",
    "HeLa cells",
    "cell clustering",
    "aggregation kinetics",
    "tissue engineering"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3233/bir-14034
PubMed: 25759371

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