Unraveling the effects of static magnetic field stress on cytosolic proteins of Salmonella by using a proteomic approach.

Canadian journal of microbiology · 2016 · Mixed · Low evidence

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MixedLow evidencePaper 2955

Study snapshot

PublicationCanadian journal of microbiology · 2016

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

The present study investigated the adaptation of Salmonella enterica subsp. enterica serovar Hadar to static magnetic field (SMF) exposure (200 mT, 9 h). The proteomic analysis provides an overview of potentially important cytosolic proteins that Salmonella needs to regulate to survive and adapt to magnetic stress. Via 2-dimensional electrophoresis and liquid chromatography tandem mass spectrometry, we compared cytosolic proteomes before and after exposure to magnetic field. A total of 35 proteins displaying more than a 2-fold change were differentially expressed in exposed cells, among which 25 were upregulated and 10 were downregulated. These proteins can be classified mainly into 6 categories: (i) proteins involved in metabolic pathways of carbohydrates, (ii) chaperones and proteins produced in response to oxidative stress, (iii) proteins involved in energy homeostasis, (iv) elongation factors (EF-Tu and EF-Ts), (v) proteins involved in motility, and (vi) proteins involved in molecules transport. Many of the presented observations could be explained, while some represent still-unknown mechanisms. In addition, this study reveals 5 hypothetical proteins. It seems that the stress response to SMF (200 mT) is essentially set up to avoid oxidative damages, with the overexpression of proteins directly involved in oxidative stress response and metabolic switches to counteract oxidative stress. Interestingly, several proteins induced under SMF exposure are found to overlap with those induced by other stresses, such as heat shock and starvation.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationSalmonella enterica subsp. enterica serovar Hadar (cytosolic proteins)

Sample sizeNot supplied

Exposurestatic · 9 h

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

After exposure to a 200 mT static magnetic field for 9 h, 35 cytosolic proteins showed >2-fold differential expression (25 upregulated, 10 downregulated) compared with pre-exposure. Differentially expressed proteins were mainly related to carbohydrate metabolism, oxidative stress response/chaperones, energy homeostasis, elongation factors, motility, and transport; the authors suggest the response is oriented toward avoiding oxidative damage.

Outcomes measured

Differential cytosolic protein expression (2D electrophoresis; LC-MS/MS)
Upregulation/downregulation of proteins across functional categories (metabolism, oxidative stress response, energy homeostasis, elongation factors, motility, transport)
Identification of hypothetical proteins

Limitations

No sample size or replication details provided in the abstract
Outcomes are proteomic changes; no direct measures of viability, growth, or pathogenicity reported in the abstract
Mechanistic interpretation is partly uncertain ("still-unknown mechanisms")

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "static",
    "source": "static magnetic field (SMF) exposure",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "9 h"
  },
  "population": "Salmonella enterica subsp. enterica serovar Hadar (cytosolic proteins)",
  "sample_size": null,
  "outcomes": [
    "Differential cytosolic protein expression (2D electrophoresis; LC-MS/MS)",
    "Upregulation/downregulation of proteins across functional categories (metabolism, oxidative stress response, energy homeostasis, elongation factors, motility, transport)",
    "Identification of hypothetical proteins"
  ],
  "main_findings": "After exposure to a 200 mT static magnetic field for 9 h, 35 cytosolic proteins showed >2-fold differential expression (25 upregulated, 10 downregulated) compared with pre-exposure. Differentially expressed proteins were mainly related to carbohydrate metabolism, oxidative stress response/chaperones, energy homeostasis, elongation factors, motility, and transport; the authors suggest the response is oriented toward avoiding oxidative damage.",
  "effect_direction": "mixed",
  "limitations": [
    "No sample size or replication details provided in the abstract",
    "Outcomes are proteomic changes; no direct measures of viability, growth, or pathogenicity reported in the abstract",
    "Mechanistic interpretation is partly uncertain (\"still-unknown mechanisms\")"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "static magnetic field",
    "SMF",
    "200 mT",
    "Salmonella enterica",
    "proteomics",
    "2-dimensional electrophoresis",
    "LC-MS/MS",
    "oxidative stress",
    "chaperones",
    "differential expression"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1139/cjm-2015-0532
PubMed: 26928316

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