Effects of radiofrequency radiation in the presence of gold nanoparticles for the treatment of renal cell carcinoma.

Journal of renal injury prevention · 2017 · Mixed · Very low evidence

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MixedVery low evidencePaper 2865

Study snapshot

PublicationJournal of renal injury prevention · 2017

Study typein_vitro

Reported directionMixed

Evidence ratingVery low

Abstract

The most common type of kidney cancer is renal cell carcinoma (RCC), which accounts for more than 80% of all kidney cancers. The aim of this study was to evaluate the effects of radiofrequency (RF) radiation in the presence of gold nanoparticles (GNPs) for the treatment of RCC. Human embryonic kidney (HEK) cancer cells were divided into 6 groups. Various tests were performed on HEK cells in the presence of RF and GNPs. In order to investigate the radiation effects on the cells' survival, MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-iphenyltetrazolium bromide] assay was performed at different days during and post-irradiation period. The repeated measure analysis of variance (ANOVA) method was used for statistical analysis of the cells' survival using SPSS version 16.0. A significant level of 0.05 was considered to the tests. Using the ANOVA test, a significant decrease in cell's survival was seen in the RF exposed group 3 compared to the control group (=0.035). While, differences were not significant between RF exposed group 2 and the control group (>0.05). A significant decrease in cell's survival in the RF exposed groups 5 (=0.025) and 6 (=0.018) at the presence of GNP compared to the control group was seen. Results of this study showed that, this method can be efficiently used for RCC treatment as an alternative to nephrectomy. More follow up in vivo studies on mammalians are needed to investigate the potential of the presented method for clinical applications.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman embryonic kidney (HEK) cancer cells (renal cell carcinoma model)

Sample sizeNot supplied

ExposureRF

Evidence strengthVery lowConfidence: 74% · Peer reviewed: yes

Main findings

HEK cancer cells were divided into 6 groups and assessed for survival using MTT during and after RF irradiation, with and without gold nanoparticles (GNPs). A significant decrease in cell survival was reported for one RF-exposed group (group 3) versus control (p=0.035), while another RF-exposed group (group 2) did not differ significantly from control (p>0.05). In the presence of GNPs, significant decreases in survival were reported for RF-exposed groups 5 (p=0.025) and 6 (p=0.018) versus control.

Outcomes measured

Cell survival (MTT assay)

Limitations

In vitro study (cell culture) with no in vivo/clinical outcomes
RF exposure parameters (e.g., frequency, SAR, duration) not reported in the abstract
Sample size and detailed group definitions not provided in the abstract
Authors note need for follow-up in vivo mammalian studies

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human embryonic kidney (HEK) cancer cells (renal cell carcinoma model)",
  "sample_size": null,
  "outcomes": [
    "Cell survival (MTT assay)"
  ],
  "main_findings": "HEK cancer cells were divided into 6 groups and assessed for survival using MTT during and after RF irradiation, with and without gold nanoparticles (GNPs). A significant decrease in cell survival was reported for one RF-exposed group (group 3) versus control (p=0.035), while another RF-exposed group (group 2) did not differ significantly from control (p>0.05). In the presence of GNPs, significant decreases in survival were reported for RF-exposed groups 5 (p=0.025) and 6 (p=0.018) versus control.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study (cell culture) with no in vivo/clinical outcomes",
    "RF exposure parameters (e.g., frequency, SAR, duration) not reported in the abstract",
    "Sample size and detailed group definitions not provided in the abstract",
    "Authors note need for follow-up in vivo mammalian studies"
  ],
  "evidence_strength": "very_low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radiofrequency",
    "RF radiation",
    "gold nanoparticles",
    "GNP",
    "renal cell carcinoma",
    "RCC",
    "human embryonic kidney cells",
    "HEK",
    "MTT assay",
    "cell survival",
    "in vitro"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.15171/jrip.2017.20
PubMed: 28497084

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