Efficient DNA ligation by selective heating of DNA ligase with a radio frequency alternating magnetic field.

Biochemistry and biophysics reports · 2016 · Benefit · Low evidence

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BenefitLow evidencePaper 2847

An RF alternating magnetic field caused ferromagnetic particles to dissipate heat, selectively heating and activating immobilized T4 DNA ligase with little influence on annealing of DNA ends, increasing ligation efficiency. Ligation efficiency increased with …

Study snapshot

PublicationBiochemistry and biophysics reports · 2016

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

We present a simple method for efficient DNA ligation utilizing the heat generation of ferromagnetic particles subjected to an ac magnetic field. We carry out the ligation of DNA fragments with cohesive ends using T4 DNA ligase immobilized on the surface of ferromagnetic particles. When a radio frequency alternating magnetic field is applied, ferromagnetic particles dissipate heat and DNA ligase on the particles is selectively heated up and activated with little influence on the annealing of DNA ends, as a result of which the ligation efficiency increases. We show that the ligation efficiency increases with an increase in the field amplitude.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationNot supplied

Sample sizeNot supplied

ExposureRF

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

DNA ligation efficiency
Selective heating/activation of T4 DNA ligase immobilized on ferromagnetic particles
Effect of field amplitude on ligation efficiency

Limitations

No RF frequency, exposure duration, or quantitative exposure metrics (e.g., SAR) reported in the abstract
In vitro/biochemical system; not a health outcome study
Sample size and statistical details not provided in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": "alternating magnetic field (radio frequency) applied to ferromagnetic particles",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "DNA ligation efficiency",
    "Selective heating/activation of T4 DNA ligase immobilized on ferromagnetic particles",
    "Effect of field amplitude on ligation efficiency"
  ],
  "main_findings": "An RF alternating magnetic field caused ferromagnetic particles to dissipate heat, selectively heating and activating immobilized T4 DNA ligase with little influence on annealing of DNA ends, increasing ligation efficiency. Ligation efficiency increased with increasing field amplitude.",
  "effect_direction": "benefit",
  "limitations": [
    "No RF frequency, exposure duration, or quantitative exposure metrics (e.g., SAR) reported in the abstract",
    "In vitro/biochemical system; not a health outcome study",
    "Sample size and statistical details not provided in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radio frequency",
    "alternating magnetic field",
    "ferromagnetic particles",
    "selective heating",
    "T4 DNA ligase",
    "DNA ligation",
    "field amplitude"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.bbrep.2016.10.006
PubMed: 28955977

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