The effect of Wi-Fi on elastic and collagen fibres in the blood vessel wall of the chorioallantoic membrane

Acta Vet. Brno · 2025 · Mixed · Low evidence

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MixedLow evidencePaper 2516

Study snapshot

PublicationActa Vet. Brno · 2025

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

Category: Experimental Biology Tags: Wi-Fi, electromagnetic fields, collagen, elastic fibers, blood vessel wall, chorioallantoic membrane, cardiovascular risk DOI: 10.2754/avb202594020137 URL: actavet.vfu.cz Overview This study investigates the impact of Wi-Fi radiation on the structural fibers that compose large blood vessel walls in the chorioallantoic membrane (CAM) of chicken embryos. Experimental groups were exposed continuously (24 hours per day) to Wi-Fi at a frequency of 2.4 GHz and an average power density of 300 μW/m2 for either 9 or 14 embryonic days. Findings - Traditional haematoxylin and eosin staining did not reveal significant structural differences in large blood vessel walls between control and Wi-Fi-exposed embryos. - Special staining techniques, however, demonstrated marked changes in fibrous vessel wall components after Wi-Fi exposure: - The optical density (OD) of elastic fibers significantly decreased from 0.294 ± 0.025 (control) to 0.197 ± 0.022 (Wi-Fi) at day 9, and from 0.706 ± 0.028 (control) to 0.271 ± 0.031 (Wi-Fi) at day 14. - The OD of collagen fibers increased at day 9 (from 0.178 ± 0.023 to 0.334 ± 0.020), but showed a significant decrease by day 14 (from 0.418 ± 0.033 to 0.327 ± 0.031). Conclusion The results of this open access study indicate that Wi-Fi can alter the balance and composition of fibrous elements in the large vessel wall, potentially playing a role in the development of cardiovascular disorders. This supports concerns that electromagnetic fields such as those from Wi-Fi may adversely affect biological tissue and vascular health.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionmixed

PopulationChicken embryos (chorioallantoic membrane, CAM)

Sample sizeNot supplied

ExposureRF · 2400 MHz · Continuous (24 h/day) exposure for 9 or 14 embryonic days

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Continuous 2.4 GHz Wi‑Fi exposure (average power density 300 μW/m²) did not show significant differences in large vessel wall structure on H&E staining versus controls. Special stains showed decreased optical density of elastic fibers at embryonic day 9 and day 14 in exposed embryos. Collagen fiber optical density increased at day 9 but decreased by day 14 compared with controls.

Outcomes measured

Histological structure of large blood vessel wall (H&E staining)
Optical density of elastic fibers in vessel wall
Optical density of collagen fibers in vessel wall

Limitations

Sample size not reported in the provided abstract/metadata
Exposure metric reported as average power density; SAR not reported
Only histological/optical density endpoints reported in the abstract; functional cardiovascular outcomes not described
Details of randomization/blinding and statistical methods not provided in the abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "RF",
    "source": "wi-fi",
    "frequency_mhz": 2400,
    "sar_wkg": null,
    "duration": "Continuous (24 h/day) exposure for 9 or 14 embryonic days"
  },
  "population": "Chicken embryos (chorioallantoic membrane, CAM)",
  "sample_size": null,
  "outcomes": [
    "Histological structure of large blood vessel wall (H&E staining)",
    "Optical density of elastic fibers in vessel wall",
    "Optical density of collagen fibers in vessel wall"
  ],
  "main_findings": "Continuous 2.4 GHz Wi‑Fi exposure (average power density 300 μW/m²) did not show significant differences in large vessel wall structure on H&E staining versus controls. Special stains showed decreased optical density of elastic fibers at embryonic day 9 and day 14 in exposed embryos. Collagen fiber optical density increased at day 9 but decreased by day 14 compared with controls.",
  "effect_direction": "mixed",
  "limitations": [
    "Sample size not reported in the provided abstract/metadata",
    "Exposure metric reported as average power density; SAR not reported",
    "Only histological/optical density endpoints reported in the abstract; functional cardiovascular outcomes not described",
    "Details of randomization/blinding and statistical methods not provided in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "Wi-Fi",
    "2.4 GHz",
    "radiofrequency",
    "electromagnetic fields",
    "power density",
    "chorioallantoic membrane",
    "chicken embryo",
    "blood vessel wall",
    "elastic fibers",
    "collagen fibers",
    "histology"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.2754/avb202594020137
PubMed: Not supplied

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