Electric Fields and Enzyme Catalysis

2017 · Unclear · Insufficient evidence

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UnclearInsufficient evidencePaper 2307

The review describes recent experiments using the vibrational Stark effect to measure electric fields experienced by substrates in enzyme active sites and states these experiments provide evidence supporting a major electrostatic contribution to enzymatic cat…

Study snapshot

Publicationmanual · 2017

Study typereview

Reported directionUnclear

Evidence ratingInsufficient

Abstract

Electric Fields and Enzyme Catalysis Fried SD, Boxer SG. Electric Fields and Enzyme Catalysis. Annu Rev Biochem. 2017 Mar 24. doi: 10.1146/annurev- biochem-061516-044432 Abstract What happens inside an enzyme's active site to allow slow and difficult chemical reactions to occur so rapidly? This question has occupied biochemists' attention for a long time. Computer models of increasing sophistication have predicted an important role for electrostatic interactions in enzymatic reactions, yet this hypothesis has proved vexingly difficult to test experimentally. Recent experiments utilizing the vibrational Stark effect make it possible to measure the electric field a substrate molecule experiences when bound inside its enzyme's active site. These experiments have provided compelling evidence supporting a major electrostatic contribution to enzymatic catalysis. Here, we review these results and develop a simple model for electrostatic catalysis that enables us to incorporate disparate concepts introduced by many investigators to describe how enzymes work into a more unified framework stressing the importance of electric fields at the active site. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typereview

Effect directionunclear

PopulationNot supplied

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthInsufficientConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

electrostatic contribution to enzymatic catalysis
electric field measurements in enzyme active sites (vibrational Stark effect)
model for electrostatic catalysis

View raw extracted JSON

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  "study_type": "review",
  "exposure": {
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  },
  "population": null,
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  "outcomes": [
    "electrostatic contribution to enzymatic catalysis",
    "electric field measurements in enzyme active sites (vibrational Stark effect)",
    "model for electrostatic catalysis"
  ],
  "main_findings": "The review describes recent experiments using the vibrational Stark effect to measure electric fields experienced by substrates in enzyme active sites and states these experiments provide evidence supporting a major electrostatic contribution to enzymatic catalysis.",
  "effect_direction": "unclear",
  "limitations": [],
  "evidence_strength": "insufficient",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "enzyme catalysis",
    "electric fields",
    "electrostatics",
    "active site",
    "vibrational Stark effect",
    "Stark effect",
    "electrostatic catalysis"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1146/annurev
PubMed: Not supplied

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