Biophysical control of the growth of Agrobacterium tumefaciens using ELF EMF at resonance frequency

2017 · Harm · Low evidence

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HarmLow evidencePaper 2207

Study snapshot

Publicationmanual · 2017

Study typeanimal

Reported directionHarm

Evidence ratingLow

Abstract

Biophysical control of the growth of Agrobacterium tumefaciens using ELF EMF at resonance frequency Fadel MA, El-Gebaly RH, Mohamed SA, Abdelbacki AMM. Biophysical control of the growth of Agrobacterium tumefaciens using extremely low frequency electromagnetic waves at resonance frequency. Biochem Biophys Res Commun. 2017 Dec 9;494(1-2):365-371. Abstract Isolated Agrobacterium tumefaciens was exposed to different extremely low frequencies of square amplitude modulated waves (QAMW) from two generators to determine the resonance frequency that causes growth inhibition. The carrier was 10 MHz sine wave with amplitude ±10 Vpp which was modulated by a second wave generator with a modulation depth of ± 2Vpp and constant field strength of 200 V/m at 28 °C. The exposure of A. tumefaciens to 1.0 Hz QAMW for 90 min inhibited the bacterial growth by 49.2%. In addition, the tested antibiotics became more effective against A. tumefaciens after the exposure. Furthermore, results of DNA, dielectric relaxation and TEM showed highly significant molecular and morphological changes due to the exposure to 1.0 Hz QAMW for 90 min. An in-vivo study has been carried out on healthy tomato plants to test the pathogenicity of A. tumefaciens before and after the exposure to QAMW at the inhibiting frequency. Symptoms of crown gall and all pathological symptoms were more aggressive in tomato plants treated with non-exposed bacteria, comparing with those treated with exposed bacteria. We concluded that, the exposure of A. tumefaciens to 1.0 Hz QAMW for 90 min modified its cellular activity and DNA structure, which inhibited the growth and affected the microbe pathogenicity. ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionharm

PopulationAgrobacterium tumefaciens (bacteria); in-vivo pathogenicity tested in healthy tomato plants

Sample sizeNot supplied

ExposureELF · 10 MHz · 90 min

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Exposure to square amplitude modulated waves with a 10 MHz carrier and 1.0 Hz modulation for 90 min (field strength 200 V/m, 28°C) inhibited A. tumefaciens growth by 49.2%. Antibiotics tested became more effective after exposure, and DNA, dielectric relaxation, and TEM results indicated significant molecular and morphological changes. Tomato plants inoculated with exposed bacteria showed less aggressive crown gall/pathological symptoms than plants inoculated with non-exposed bacteria.

Outcomes measured

Bacterial growth inhibition
Antibiotic effectiveness against A. tumefaciens
DNA changes
Dielectric relaxation changes
Ultrastructural/morphological changes (TEM)
Pathogenicity in tomato plants (crown gall/aggressiveness of symptoms)

Limitations

Sample size not reported in abstract
Exposure metrics such as SAR not reported
Details of antibiotics tested and quantitative results not provided in abstract
In-vivo tomato study design details (randomization/blinding/replicates) not described

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": 10,
    "sar_wkg": null,
    "duration": "90 min"
  },
  "population": "Agrobacterium tumefaciens (bacteria); in-vivo pathogenicity tested in healthy tomato plants",
  "sample_size": null,
  "outcomes": [
    "Bacterial growth inhibition",
    "Antibiotic effectiveness against A. tumefaciens",
    "DNA changes",
    "Dielectric relaxation changes",
    "Ultrastructural/morphological changes (TEM)",
    "Pathogenicity in tomato plants (crown gall/aggressiveness of symptoms)"
  ],
  "main_findings": "Exposure to square amplitude modulated waves with a 10 MHz carrier and 1.0 Hz modulation for 90 min (field strength 200 V/m, 28°C) inhibited A. tumefaciens growth by 49.2%. Antibiotics tested became more effective after exposure, and DNA, dielectric relaxation, and TEM results indicated significant molecular and morphological changes. Tomato plants inoculated with exposed bacteria showed less aggressive crown gall/pathological symptoms than plants inoculated with non-exposed bacteria.",
  "effect_direction": "harm",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure metrics such as SAR not reported",
    "Details of antibiotics tested and quantitative results not provided in abstract",
    "In-vivo tomato study design details (randomization/blinding/replicates) not described"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "Agrobacterium tumefaciens",
    "extremely low frequency",
    "ELF EMF",
    "resonance frequency",
    "square amplitude modulated waves",
    "QAMW",
    "10 MHz carrier",
    "1.0 Hz modulation",
    "growth inhibition",
    "DNA",
    "dielectric relaxation",
    "TEM",
    "tomato plants",
    "crown gall",
    "pathogenicity"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: Not supplied

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