1950 MHz RF EMR Inhibits Testosterone Secretion of Mouse Leydig Cells

Environ Res Public Health. · 2017 · Harm · Low evidence

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HarmLow evidencePaper 2176

24 hours of 1950 MHz RF radiation at 3 W/kg reduced cell proliferation, cell cycle distribution, testosterone secretion, and P450scc mRNA levels in TM3 cells. Apoptosis, ROS, and StAR mRNA levels did not change significantly.

Study snapshot

PublicationEnviron Res Public Health. · 2017

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

1950 MHz RF EMR Inhibits Testosterone Secretion of Mouse Leydig Cells Lin YY, Wu T, Liu JY, Gao P, Li KC, Guo QY, Yuan M, Lang HY, Zeng LH, Guo GZ. 1950 MHz Radio Frequency Electromagnetic Radiation Inhibits Testosterone Secretion of Mouse Leydig Cells. Int J Environ Res Public Health. 2017 Dec 23;15(1). pii: E17. doi: 10.3390/ijerph15010017. Abstract More studies that are focused on the bioeffects of radio-frequency (RF) electromagnetic radiation that is generated from the communication devices, but there were few reports with confirmed results about the bioeffects of RF radiation on reproductive cells. To explore the effects of 1950 MHz RF electromagnetic radiation (EMR) on mouse Leydig (TM3) cells. TM3 cells were irradiated or sham-irradiated continuously for 24 h by the specific absorption rate (SAR) 3 W/kg radiation. At 0, 1, 2, 3, 4, and 5 days after irradiation, cell proliferation was detected by cell counting kit-8 (CCK-8) method, cell cycle distribution, percentage of apoptosis, and cellular reactive oxygen species (ROS) were examined by flow cytometry, Testosterone level was measured using enzyme-linked immunosorbent assay (ELISA) assay, messenger ribonucleic acid (mRNA) expression level of steroidogenic acute regulatory protein (StAR) and P450scc in TM3 cells was detected by real-time polymerase chain reaction (PCR). After being irradiated for 24 h, cell proliferation obviously decreased and cell cycle distribution, secretion capacity of Testosterone, and P450scc mRNA level were reduced. While cell apoptosis, ROS, and StAR mRNA level did not change significantly. The current results indicated that 24 h of exposure at 1950 MHz 3 W/kg radiation could cause some adverse effects on TM3 cells proliferation and Testosterone secretion, further studies about the biological effects in the reproductive system that are induced by RF radiation are also needed. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

Populationmouse Leydig (TM3) cells

Sample sizeNot supplied

ExposureRF · 1950 MHz · 3 W/kg · 24 hours continuous

Evidence strengthLowConfidence: 60% · Peer reviewed: yes

Main findings

Outcomes measured

cell proliferation
cell cycle distribution
percentage of apoptosis
cellular reactive oxygen species (ROS)
testosterone secretion
mRNA expression of steroidogenic acute regulatory protein (StAR)
mRNA expression of P450scc

Limitations

in vitro study, results may not translate directly to in vivo or human effects
single cell type studied
only one exposure duration and SAR level tested

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 1950,
    "sar_wkg": 3,
    "duration": "24 hours continuous"
  },
  "population": "mouse Leydig (TM3) cells",
  "sample_size": null,
  "outcomes": [
    "cell proliferation",
    "cell cycle distribution",
    "percentage of apoptosis",
    "cellular reactive oxygen species (ROS)",
    "testosterone secretion",
    "mRNA expression of steroidogenic acute regulatory protein (StAR)",
    "mRNA expression of P450scc"
  ],
  "main_findings": "24 hours of 1950 MHz RF radiation at 3 W/kg reduced cell proliferation, cell cycle distribution, testosterone secretion, and P450scc mRNA levels in TM3 cells. Apoptosis, ROS, and StAR mRNA levels did not change significantly.",
  "effect_direction": "harm",
  "limitations": [
    "in vitro study, results may not translate directly to in vivo or human effects",
    "single cell type studied",
    "only one exposure duration and SAR level tested"
  ],
  "evidence_strength": "low",
  "confidence": 0.6,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radio-frequency electromagnetic radiation",
    "Leydig cells",
    "testosterone secretion",
    "cell proliferation",
    "oxidative stress",
    "steroidogenic acute regulatory protein",
    "P450scc"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3390/ijerph15010017
PubMed: Not supplied

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