Global gene expression analysis of Escherichia coli K-12 DH5α after exposure to 2.4 GHz wireless fidelity

2019 · Mixed · Low evidence

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MixedLow evidencePaper 1678

Study snapshot

Publicationmanual · 2019

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Global gene expression analysis of Escherichia coli K-12 DH5α after exposure to 2.4 GHz wireless fidelity radiation Said-Salman IH, Jebaii FA, Yusef HH, Moustafa ME. Global gene expression analysis of Escherichia coli K-12 DH5α after exposure to 2.4 GHz wireless fidelity radiation. Sci Rep. 2019 Oct 8;9(1):14425. doi: 10.1038/s41598-019- 51046-7. Abstract This study investigated the non-thermal effects of Wi-Fi radiofrequency radiation of 2.4 GHz on global gene expression in Escherichia coli K-12 DH5α. High-throughput RNA-sequencing of 2.4 GHz exposed and non- exposed bacteria revealed that 101 genes were differentially expressed (DEGs) at P ≤ 0.05. The up-regulated genes were 52 while the down-regulated ones were 49. QRT-PCR analysis of pgaD, fliC, cheY, malP, malZ, motB, alsC, alsK, appB and appX confirmed the RNA-seq results. About 7% of DEGs are involved in cellular component organization, 6% in response to stress stimulus, 6% in biological regulation, 6% in localization, 5% in locomotion and 3% in cell adhesion. Database for annotation, visualization and integrated discovery (DAVID) functional clustering revealed that DEGs with high enrichment score included genes for localization of cell, locomotion, chemotaxis, response to external stimulus and cell adhesion. Kyoto encyclopedia of genes and genomes (KEGG) pathways analysis showed that the pathways for flagellar assembly, chemotaxis and two-component system were affected. Go enrichment analysis indicated that the up-regulated DEGs are involved in metabolic pathways, transposition, response to stimuli, motility, chemotaxis and cell adhesion. The down-regulated DEGs are associated with metabolic pathways and localization of ions and organic molecules. Therefore, the exposure of E. coli DH5α to Wi-Fi radiofrequency radiation for 5 hours influenced several bacterial cellular and metabolic processes. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationEscherichia coli K-12 DH5α

Sample sizeNot supplied

ExposureRF · 2400 MHz · 5 hours

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

RNA-sequencing comparing 2.4 GHz Wi-Fi-exposed vs non-exposed E. coli identified 101 differentially expressed genes (P ≤ 0.05), with 52 up-regulated and 49 down-regulated. Enrichment and pathway analyses indicated effects on processes/pathways including localization, locomotion/chemotaxis, response to external stimulus, cell adhesion, and flagellar assembly; the authors conclude 5-hour exposure influenced several cellular and metabolic processes.

Outcomes measured

Global gene expression (RNA-seq)
Differentially expressed genes (DEGs)
qRT-PCR confirmation of selected genes
Functional enrichment/clustering (DAVID, GO)
KEGG pathway changes (flagellar assembly, chemotaxis, two-component system)

Limitations

Exposure metrics beyond frequency and duration (e.g., power density/SAR) not reported in the abstract
Sample size/replication details not reported in the abstract
Statistical/multiple-testing correction approach not described in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": "wi-fi",
    "frequency_mhz": 2400,
    "sar_wkg": null,
    "duration": "5 hours"
  },
  "population": "Escherichia coli K-12 DH5α",
  "sample_size": null,
  "outcomes": [
    "Global gene expression (RNA-seq)",
    "Differentially expressed genes (DEGs)",
    "qRT-PCR confirmation of selected genes",
    "Functional enrichment/clustering (DAVID, GO)",
    "KEGG pathway changes (flagellar assembly, chemotaxis, two-component system)"
  ],
  "main_findings": "RNA-sequencing comparing 2.4 GHz Wi-Fi-exposed vs non-exposed E. coli identified 101 differentially expressed genes (P ≤ 0.05), with 52 up-regulated and 49 down-regulated. Enrichment and pathway analyses indicated effects on processes/pathways including localization, locomotion/chemotaxis, response to external stimulus, cell adhesion, and flagellar assembly; the authors conclude 5-hour exposure influenced several cellular and metabolic processes.",
  "effect_direction": "mixed",
  "limitations": [
    "Exposure metrics beyond frequency and duration (e.g., power density/SAR) not reported in the abstract",
    "Sample size/replication details not reported in the abstract",
    "Statistical/multiple-testing correction approach not described in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "Wi-Fi",
    "2.4 GHz",
    "radiofrequency radiation",
    "non-thermal effects",
    "Escherichia coli",
    "RNA-seq",
    "gene expression",
    "qRT-PCR",
    "DAVID",
    "GO enrichment",
    "KEGG pathways",
    "chemotaxis",
    "flagellar assembly"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1038/s41598-019
PubMed: Not supplied

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