RF Safe

Immunotropic effects in cultured human blood mononuclear cells exposed to a 900 MHz pulse-modulated microwave field

2019 · Mixed · Low evidence

← Research stream
MixedLow evidencePaper 1637

PBMC exposed to 900 MHz pulse-modulated RF showed higher monocyte immunogenic activity (LM index) and higher T-cell response to concanavalin A than controls after the first exposure, but these parameters decreased after a second stimulation. IL-2 receptor sat…

Study snapshot

Publicationmanual · 2019
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

Immunotropic effects in cultured human blood mononuclear cells exposed to a 900 MHz pulse-modulated microwave field Szymański Ł, Sobiczewska E, Cios A, Szymanski P, Ciepielak M, Stankiewicz W. Immunotropic effects in cultured human blood mononuclear cells exposed to a 900 MHz pulse-modulated microwave field. J Radiat Res. 2019 Dec 12. pii: rrz085. doi: 10.1093/jrr/rrz085. Abstract The specific biological effect of electromagnetic field (EMF) remains unknown even though devices present in our daily lives, such as smartphones and Wi-Fi antennae increase the environmental level of electromagnetic radiation. It is said that the human immune system is able to react to discrete environmental stimuli like EMF. To investigate the effect of 900 MHz microwave stimulation on the immune system our research aimed to analyze lymphocyte proliferation and observe and assess the basic immunoregulatory activities using a newly developed and improved anechoic chamber. Samples of mononuclear cells (PBMC) isolated from the blood of healthy donors were exposed to 900 MHz pulse-modulated radiofrequency radiation (20 V/m, SAR 0.024 W/kg) twice (15 min each) or left without irradiation (control group). Subsequently, the control and exposed cells were set up to determine several parameters characterizing T cell immunocompetence and monocyte immunogenic activity. Although the microcultures of PBMC exposed to radiofrequency radiation demonstrated higher immunogenic activity of monocytes (LM index) and T-cell response to concanavalin A than control cultures after first exposure, this parameter decreased after a second stimulation. Saturation of the interleukin-2 (IL-2) receptor rose significantly after the second day of exposure. On the other hand, response to mitogen dropped after EMF stimulation. The results suggest that PBMC are able to overcome stress caused by mitogens after stimulation with 900 MHz radiation. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationCultured peripheral blood mononuclear cells (PBMC) isolated from blood of healthy donors
Sample sizeNot supplied
ExposureRF · 900 MHz · 0.024 W/kg · Two exposures of 15 min each (pulse-modulated; 20 V/m)
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

PBMC exposed to 900 MHz pulse-modulated RF showed higher monocyte immunogenic activity (LM index) and higher T-cell response to concanavalin A than controls after the first exposure, but these parameters decreased after a second stimulation. IL-2 receptor saturation increased significantly after the second day of exposure, while response to mitogen dropped after EMF stimulation.

Outcomes measured

  • Lymphocyte proliferation
  • T-cell response to concanavalin A
  • Monocyte immunogenic activity (LM index)
  • Interleukin-2 (IL-2) receptor saturation
  • Response to mitogen

Limitations

  • In vitro study in cultured PBMC; generalizability to in vivo human health outcomes is unclear
  • Sample size and donor characteristics not reported in the abstract
  • Exposure regimen limited to short, twice-15-minute exposures
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 900,
    "sar_wkg": 0.024,
    "duration": "Two exposures of 15 min each (pulse-modulated; 20 V/m)"
  },
  "population": "Cultured peripheral blood mononuclear cells (PBMC) isolated from blood of healthy donors",
  "sample_size": null,
  "outcomes": [
    "Lymphocyte proliferation",
    "T-cell response to concanavalin A",
    "Monocyte immunogenic activity (LM index)",
    "Interleukin-2 (IL-2) receptor saturation",
    "Response to mitogen"
  ],
  "main_findings": "PBMC exposed to 900 MHz pulse-modulated RF showed higher monocyte immunogenic activity (LM index) and higher T-cell response to concanavalin A than controls after the first exposure, but these parameters decreased after a second stimulation. IL-2 receptor saturation increased significantly after the second day of exposure, while response to mitogen dropped after EMF stimulation.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study in cultured PBMC; generalizability to in vivo human health outcomes is unclear",
    "Sample size and donor characteristics not reported in the abstract",
    "Exposure regimen limited to short, twice-15-minute exposures"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "900 MHz",
    "pulse-modulated",
    "radiofrequency radiation",
    "microwave field",
    "PBMC",
    "mononuclear cells",
    "immune system",
    "T cells",
    "monocytes",
    "IL-2 receptor",
    "anechoic chamber",
    "SAR 0.024 W/kg",
    "20 V/m"
  ],
  "suggested_hubs": []
}
AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1093/jrr/rrz085
PubMed: Not supplied

Read the original paper →

Related papers

← Back to the Research Library