In vitro study of effects of ELF-EMF on testicular tissues of roe

2020 · Harm · Low evidence

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HarmLow evidencePaper 1620

Testicular tissues exposed in vitro to ELF-EMF (50 or 120 Hz; magnetic induction 8 mT; 2 or 4 h) showed characteristic spectral differences versus controls by FTIR and FT-Raman, including increased intensity of peaks from most functional groups and multiple p…

Study snapshot

Publicationmanual · 2020

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

In vitro study of effects of ELF-EMF on testicular tissues of roe Koziorowska A, Depciuch J, Kozioł K, Nowak S, Lach K. In vitro study of effects of ELF-EMF on testicular tissues of roe deer (Capreolus capreolus) - FTIR and FT-Raman spectroscopic investigation. Anim Reprod Sci. 2020 Feb;213:106258. doi: 10.1016/j.anireprosci.2019.106258. Abstract Electromagnetic fields (EMF) are classified as an environmental factor affecting living organisms. The aim of the study was to evaluate the effect of EMF at different frequencies (50 and 120 Hz), durations of treatment (2 and 4 h) and with the magnetic induction of 8 m T on testicular tissues of roe deer (Capreolus capreolus) in vitro by comparison with the control samples. Fourier Transform Raman Spectroscopy (FT-Raman) and Fourier Transform Infrared Spectroscopy (FTIR) were utilized in this study to identify the chemical changes in the testicular tissues. The FTIR and FT-Raman spectroscopy methods were used to evaluate differences in spectra of the treated tissues compared to the control group. The results from the analysis of the spectra indicated there were characteristic differences in the testicular tissue compared with the control samples. There was identification of peaks attributed to different biochemical components. Comparing the spectra for different frequencies and treatment times, there was a greater intensity of peaks originating from most of the functional groups in the tissues evaluated. With the FTIR spectra, there were five of 15 peaks, while with the FT-Raman spectra, there were six of ten peaks that were shifted. For FTIR and FT-Raman analyzed spectral ranges, results from the PCA analysis indicate there was no similarity between control groups (2 and 4 h) and samples treated with EMF at a frequency of 120 Hz for 2 and 4 h. In conclusion, therefore, EMF is an environmental factor affecting the testis of roe deer. ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

PopulationRoe deer (Capreolus capreolus) testicular tissue (in vitro)

Sample sizeNot supplied

ExposureELF · 2 h and 4 h

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

FTIR spectral changes in testicular tissue
FT-Raman spectral changes in testicular tissue
Biochemical/chemical composition changes (spectral peak intensity/shift)
PCA separation/similarity vs control

Limitations

In vitro tissue study; findings may not generalize to whole organisms or in vivo exposure
Sample size not reported in provided abstract/metadata
Outcomes are spectroscopic/biochemical markers; no functional fertility or histopathology endpoints described
Exposure conditions limited to 50/120 Hz, 8 mT, and 2–4 h durations

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "2 h and 4 h"
  },
  "population": "Roe deer (Capreolus capreolus) testicular tissue (in vitro)",
  "sample_size": null,
  "outcomes": [
    "FTIR spectral changes in testicular tissue",
    "FT-Raman spectral changes in testicular tissue",
    "Biochemical/chemical composition changes (spectral peak intensity/shift)",
    "PCA separation/similarity vs control"
  ],
  "main_findings": "Testicular tissues exposed in vitro to ELF-EMF (50 or 120 Hz; magnetic induction 8 mT; 2 or 4 h) showed characteristic spectral differences versus controls by FTIR and FT-Raman, including increased intensity of peaks from most functional groups and multiple peak shifts. PCA indicated no similarity between control samples and samples treated at 120 Hz for 2 and 4 h.",
  "effect_direction": "harm",
  "limitations": [
    "In vitro tissue study; findings may not generalize to whole organisms or in vivo exposure",
    "Sample size not reported in provided abstract/metadata",
    "Outcomes are spectroscopic/biochemical markers; no functional fertility or histopathology endpoints described",
    "Exposure conditions limited to 50/120 Hz, 8 mT, and 2–4 h durations"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "120 Hz",
    "8 mT",
    "magnetic induction",
    "testis",
    "testicular tissue",
    "roe deer",
    "Capreolus capreolus",
    "in vitro",
    "FTIR",
    "FT-Raman",
    "spectroscopy",
    "PCA"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.anireprosci.2019.106258
PubMed: Not supplied

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