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The inverse relation between mitochondrial transmembrane potential and proteins α-helix in neuronal-like cells under static magnetic field and the role of VDAC

2020 · Mixed · Low evidence

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MixedLow evidencePaper 1588

After exposure of neuronal-like cells to a uniform static magnetic field (2 mT) for 3, 7, 15, and 24 hours, the study reports a significant inverse relation between mitochondrial transmembrane potential and the intensity of the Amide I band (associated with p…

Study snapshot

Publicationmanual · 2020
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

The inverse relation between mitochondrial transmembrane potential and proteins α-helix in neuronal-like cells under static magnetic field and the role of VDAC Calabrò E, Magazù S, Currò M, Ientile R. The inverse relation between mitochondrial transmembrane potential and proteins α-helix in neuronal-like cells under static magnetic field and the role of VDAC. Electromagn Biol Med. 2020 Mar 9:1-7. doi: 10.1080/15368378.2020.1737808. Abstract In this study, a correlation between cell channel α-helices displacement and the mitochondrial transmembrane potential after exposure of 3, 7, 15 and 24 h of neuronal-like cells to a uniform magnetic field at the intensity of 2 mT was shown. Fourier Transform Infrared (FTIR) Spectroscopy and fluorescence techniques were used to analyze the secondary structure of protein content and mitochondrial transmembrane potential, respectively. The main result of this study was represented by a significant inverse relation between the mitochondrial transmembrane potential and the intensity of the Amide I band that can be associated with time exposure. Given that mitochondrial transmembrane potential should be related to the gating state of voltage-dependent anion channel (VDAC) in mitochondrial membrane, this result could have a relevant role in medicine. Indeed, VDAC's irregular behavior can be associated with several varieties of mitochondria-associated pathologies and various forms of cancer and neurodegeneration. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
Populationneuronal-like cells
Sample sizeNot supplied
ExposureELF · 3, 7, 15, and 24 h
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

After exposure of neuronal-like cells to a uniform static magnetic field (2 mT) for 3, 7, 15, and 24 hours, the study reports a significant inverse relation between mitochondrial transmembrane potential and the intensity of the Amide I band (associated with protein secondary structure) in a time-exposure-associated manner.

Outcomes measured

  • mitochondrial transmembrane potential
  • protein secondary structure (α-helix; Amide I band intensity)
  • cell channel α-helices displacement
  • VDAC-related gating state (discussed)

Limitations

  • In vitro study in neuronal-like cells; generalizability to humans not addressed in abstract
  • Sample size not reported in abstract
  • Only correlation/inverse relation described; causality not established in abstract
  • Exposure characterized only by magnetic field intensity (2 mT) and duration; other exposure parameters not described in abstract
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "static magnetic field",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "3, 7, 15, and 24 h"
  },
  "population": "neuronal-like cells",
  "sample_size": null,
  "outcomes": [
    "mitochondrial transmembrane potential",
    "protein secondary structure (α-helix; Amide I band intensity)",
    "cell channel α-helices displacement",
    "VDAC-related gating state (discussed)"
  ],
  "main_findings": "After exposure of neuronal-like cells to a uniform static magnetic field (2 mT) for 3, 7, 15, and 24 hours, the study reports a significant inverse relation between mitochondrial transmembrane potential and the intensity of the Amide I band (associated with protein secondary structure) in a time-exposure-associated manner.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study in neuronal-like cells; generalizability to humans not addressed in abstract",
    "Sample size not reported in abstract",
    "Only correlation/inverse relation described; causality not established in abstract",
    "Exposure characterized only by magnetic field intensity (2 mT) and duration; other exposure parameters not described in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "static magnetic field",
    "2 mT",
    "neuronal-like cells",
    "mitochondrial transmembrane potential",
    "FTIR spectroscopy",
    "fluorescence",
    "Amide I band",
    "alpha-helix",
    "VDAC"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/15368378.2020.1737808
PubMed: Not supplied

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