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Lipid Oxidation Induced by RF Waves and Mediated by Ferritin Iron Causes Activation of Ferritin-Tagged Ion Channels

2020 · Mixed · Low evidence

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MixedLow evidencePaper 1583

In cells expressing TRPVFeRIC channels, RF exposure increased the labile iron pool in a ferritin-dependent manner. The released iron was associated with reactive oxygen species and lipid oxidation, which ultimately activated the ferritin-tagged TRP channels.

Study snapshot

Publicationmanual · 2020
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

Lipid Oxidation Induced by RF Waves and Mediated by Ferritin Iron Causes Activation of Ferritin-Tagged Ion Channels Hernández-Morales M, Shang T, Chen J, Han V, Liu C. Lipid Oxidation Induced by RF Waves and Mediated by Ferritin Iron Causes Activation of Ferritin-Tagged Ion Channels. Cell Rep. 2020 Mar 10;30(10):3250-3260.e7. doi: 10.1016/j.celrep.2020.02.070. Abstract One approach to magnetogenetics uses radiofrequency (RF) waves to activate transient receptor potential channels (TRPV1 and TRPV4) that are coupled to cellular ferritins. The mechanisms underlying this effect are unclear and controversial. Theoretical calculations suggest that the heat produced by RF fields is likely orders of magnitude weaker than needed for channel activation. Using the FeRIC (Ferritin iron Redistribution to Ion Channels) system, we have uncovered a mechanism of activation of ferritin-tagged channels via a biochemical pathway initiated by RF disturbance of ferritin and mediated by ferritin-associated iron. We show that, in cells expressing TRPVFeRIC channels, RF increases the levels of the labile iron pool in a ferritin-dependent manner. Free iron participates in chemical reactions, producing reactive oxygen species and oxidized lipids that ultimately activate the TRPVFeRIC channels. This biochemical pathway predicts a similar RF-induced activation of other lipid-sensitive TRP channels and may guide future magnetogenetic designs. ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationNot supplied
Sample sizeNot supplied
ExposureRF
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In cells expressing TRPVFeRIC channels, RF exposure increased the labile iron pool in a ferritin-dependent manner. The released iron was associated with reactive oxygen species and lipid oxidation, which ultimately activated the ferritin-tagged TRP channels.

Outcomes measured

  • Activation of ferritin-tagged TRP ion channels (TRPV1/TRPV4; TRPVFeRIC)
  • Labile iron pool levels
  • Reactive oxygen species production
  • Lipid oxidation/oxidized lipids

Limitations

  • RF exposure parameters (e.g., frequency, SAR, duration) not provided in the abstract
  • In vitro cell-based system; generalizability to in vivo or human exposure not addressed in the abstract
  • Mechanistic claims are based on the described FeRIC system; applicability to other systems is presented as a prediction
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Activation of ferritin-tagged TRP ion channels (TRPV1/TRPV4; TRPVFeRIC)",
    "Labile iron pool levels",
    "Reactive oxygen species production",
    "Lipid oxidation/oxidized lipids"
  ],
  "main_findings": "In cells expressing TRPVFeRIC channels, RF exposure increased the labile iron pool in a ferritin-dependent manner. The released iron was associated with reactive oxygen species and lipid oxidation, which ultimately activated the ferritin-tagged TRP channels.",
  "effect_direction": "mixed",
  "limitations": [
    "RF exposure parameters (e.g., frequency, SAR, duration) not provided in the abstract",
    "In vitro cell-based system; generalizability to in vivo or human exposure not addressed in the abstract",
    "Mechanistic claims are based on the described FeRIC system; applicability to other systems is presented as a prediction"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radiofrequency",
    "RF waves",
    "magnetogenetics",
    "FeRIC",
    "ferritin",
    "TRPV1",
    "TRPV4",
    "TRP channels",
    "labile iron pool",
    "reactive oxygen species",
    "lipid oxidation"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.celrep.2020.02.070
PubMed: Not supplied

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