Influence of magnetic field on aqueous two-phase extraction of horse ferritin in the polyethylene glycol/hydroxyethyl starch system.

Analytica chimica acta · 2012 · Benefit · Low evidence

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BenefitLow evidencePaper 3256

Study snapshot

PublicationAnalytica chimica acta · 2012

Study typeother

Reported directionBenefit

Evidence ratingLow

Abstract

The presented experiments show the model of expectation of equine spleen ferritin extraction in a new aqueous two-phase system which was formed by mixing polyethylene glycol (PEG) and hydroxyethyl starch (HES). The tendency of the protein to migrate in the analyzed systems was dependent on the concentrations of HES and PEG as well as PEG molecular weight. The highest concentration of ferritin in the top phase (rich in PEG) was recorded in the system composed of 6% PEG 3000 and 3% HES. The obtained concentration was 0.88 mg mL(-1). The lowest concentration was 0.42 mg mL(-1) in the system composed of 5% PEG 6000 and 1% HES. Next the influence of the magnetic field on ferritin accumulation was analyzed. Selected samples were placed between homogeneous (S/S) or heterogeneous magnetic poles (N/S and S/N). It was observed that after the application of the magnetic field the extraction of ferritin into the PEG rich phase increased in every examined system. That increase was as high as 1.67-fold ferritin concentration in the PEG phase as compared with the total concentration of ferritin in the system before separation. Introduction of the magnetic field to two-phase extraction systems is shown as an effective method of changing the partition coefficient of ferritin.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeother

Effect directionbenefit

PopulationNot supplied

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Ferritin migration depended on PEG and HES concentrations and PEG molecular weight, with the highest ferritin concentration in the PEG-rich phase reported for 6% PEG 3000 and 3% HES (0.88 mg/mL) and the lowest for 5% PEG 6000 and 1% HES (0.42 mg/mL). When selected samples were placed between homogeneous (S/S) or heterogeneous (N/S, S/N) magnetic poles, ferritin extraction into the PEG-rich phase increased in every examined system, with increases reported up to 1.67-fold compared with the total ferritin concentration before separation.

Outcomes measured

Ferritin concentration in PEG-rich (top) phase
Ferritin partitioning/partition coefficient in PEG/HES aqueous two-phase system

Limitations

Magnetic field characteristics (e.g., field strength, frequency/time variation) not reported in the abstract
No sample size/replication details provided in the abstract
In vitro/analytical system; not a health outcome study

View raw extracted JSON

{
  "study_type": "other",
  "exposure": {
    "band": null,
    "source": "magnetic field applied during aqueous two-phase extraction (homogeneous or heterogeneous magnetic poles)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Ferritin concentration in PEG-rich (top) phase",
    "Ferritin partitioning/partition coefficient in PEG/HES aqueous two-phase system"
  ],
  "main_findings": "Ferritin migration depended on PEG and HES concentrations and PEG molecular weight, with the highest ferritin concentration in the PEG-rich phase reported for 6% PEG 3000 and 3% HES (0.88 mg/mL) and the lowest for 5% PEG 6000 and 1% HES (0.42 mg/mL). When selected samples were placed between homogeneous (S/S) or heterogeneous (N/S, S/N) magnetic poles, ferritin extraction into the PEG-rich phase increased in every examined system, with increases reported up to 1.67-fold compared with the total ferritin concentration before separation.",
  "effect_direction": "benefit",
  "limitations": [
    "Magnetic field characteristics (e.g., field strength, frequency/time variation) not reported in the abstract",
    "No sample size/replication details provided in the abstract",
    "In vitro/analytical system; not a health outcome study"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "magnetic field",
    "aqueous two-phase extraction",
    "polyethylene glycol",
    "hydroxyethyl starch",
    "ferritin",
    "partition coefficient"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.aca.2011.02.044
PubMed: 22284871

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