Extremely low-frequency electromagnetic field induces a change in proliferative capacity and redox homeostasis of human lung fibroblast cell line MRC-5

Environmental science and pollution research international · 2020 · Mixed · Low evidence

← Research stream

MixedLow evidencePaper 1470

ELF-EMF (50 Hz) exposure was reported to affect proliferative capacity and redox homeostasis in MRC-5 cells, potentially influencing cell cycle regulation via modulation of the oxidative/antioxidative defense system. Reported effects on proliferation and redo…

Study snapshot

PublicationEnvironmental science and pollution research international · 2020

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Extremely low-frequency electromagnetic field induces a change in proliferative capacity and redox homeostasis of human lung fibroblast cell line MRC-5 Maida H Lekovic, Nerkesa E Drekovic, Nihat Dz Granica, Elvis H Mahmutovic, Natasa Z Djordjevic. Extremely low- frequency electromagnetic field induces a change in proliferative capacity and redox homeostasis of human lung fibroblast cell line MRC-5. Environ Sci Pollut Res Int. 2020 Jul 10. doi: 10.1007/s11356-020-10039-0. Abstract Numerous studies have shown that extremely low-frequency electromagnetic field (ELF-EMF) by modulating oxidative-antioxidative balance in the cells achieved beneficial and harmful effects on living organisms. The aim of this study was to research changes of both proliferative capacity and redox homeostasis of human lung fibroblast cell line MRC-5 during exposure to ELF-EMF (50 Hz). The human lung fibroblast cell line MRC-5 were exposed to ELF-EMF once a day in duration of 1 h during 24 h (1 treatment 1 h/day), 48 h (2 treatments 1 h/day), 72 h (3 treatments 1 h/day), and 7 days (7 treatments 1 h/day). After 24 h of the last treatment, the proliferative capacity of the cells and the concentrations and activities of the components of the oxidative/antioxidative system were determined: superoxide anion (O2.-), hydrogen peroxide (H2O2), nitric oxide (NO), peroxynitrite (ONOO-), reduced glutathione (GSH), oxidized glutathione (GSSG), superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px), glutathione reductase (GR), and glutathione- S-transferase (GST). The results of this study show that ELF-EMF may affect a cell cycle regulation of human lung fibroblast cell line MRC-5 through modulation of oxidative/antioxidative defense system. The effects of ELF-EMF on proliferation and redox balance of human lung fibroblast cell line MRC-5 depend on exposure time. pubmed.ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman lung fibroblast cell line MRC-5

Sample sizeNot supplied

ExposureELF · 1 h/day for 24 h (1 treatment), 48 h (2 treatments), 72 h (3 treatments), or 7 days (7 treatments); outcomes assessed 24 h after last treatment

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Outcomes measured

Proliferative capacity
Cell cycle regulation (implied)
Redox/oxidative-antioxidative balance markers: superoxide anion (O2.-), hydrogen peroxide (H2O2), nitric oxide (NO), peroxynitrite (ONOO-)
Glutathione system: GSH, GSSG
Antioxidant enzymes: SOD, CAT, GSH-Px, GR, GST

Limitations

Exposure intensity/field strength not reported in abstract
No quantitative results or direction of specific biomarker changes provided in abstract
In vitro cell line model; generalizability to humans not established from abstract
Sample size and replication not reported in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "1 h/day for 24 h (1 treatment), 48 h (2 treatments), 72 h (3 treatments), or 7 days (7 treatments); outcomes assessed 24 h after last treatment"
  },
  "population": "Human lung fibroblast cell line MRC-5",
  "sample_size": null,
  "outcomes": [
    "Proliferative capacity",
    "Cell cycle regulation (implied)",
    "Redox/oxidative-antioxidative balance markers: superoxide anion (O2.-), hydrogen peroxide (H2O2), nitric oxide (NO), peroxynitrite (ONOO-)",
    "Glutathione system: GSH, GSSG",
    "Antioxidant enzymes: SOD, CAT, GSH-Px, GR, GST"
  ],
  "main_findings": "ELF-EMF (50 Hz) exposure was reported to affect proliferative capacity and redox homeostasis in MRC-5 cells, potentially influencing cell cycle regulation via modulation of the oxidative/antioxidative defense system. Reported effects on proliferation and redox balance depended on exposure time.",
  "effect_direction": "mixed",
  "limitations": [
    "Exposure intensity/field strength not reported in abstract",
    "No quantitative results or direction of specific biomarker changes provided in abstract",
    "In vitro cell line model; generalizability to humans not established from abstract",
    "Sample size and replication not reported in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "50 Hz",
    "MRC-5",
    "human lung fibroblast",
    "proliferation",
    "cell cycle",
    "oxidative stress",
    "redox homeostasis",
    "glutathione",
    "SOD",
    "catalase"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Study examines extremely low-frequency EMF exposure at 50 Hz and cellular redox/proliferation outcomes."
    }
  ]
}

AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s11356-020-10039-0
PubMed: 32651790

Read the original paper →

Related papers

← Back to the Research Library