Exposure to Electromagnetic Field during Gestation Adversely Affects the Electrophysiological Properties of Purkinje Cells in Rat Offspring

2020 · Harm · Low evidence

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HarmLow evidencePaper 1350

Male pups from dams exposed during gestation to 900 MHz pulsed RF-EMF (6 h/day) showed reduced spontaneous firing and an upward/rightward shift in the I/V curve versus controls. Peak current amplitude was significantly decreased, and reversal potential differ…

Study snapshot

Publicationmanual · 2020

Study typeanimal

Reported directionHarm

Evidence ratingLow

Abstract

Exposure to Electromagnetic Field during Gestation Adversely Affects the Electrophysiological Properties of Purkinje Cells in Rat Offspring Haghani M, Pouladvand V , Mortazavi S M J, Razavinasab M, Bayat M, Shabani M. Exposure to Electromagnetic Field during Gestation Adversely Affects the Electrophysiological Properties of Purkinje Cells in Rat Offspring. J Biomed Phys Eng. 2020 Aug 1;10(4):433-440. doi: 10.31661/jbpe.v0i0.560. Abstract Background: Prenatal adverse effects of radiofrequency electromagnetic fields (RF-EMF) exposure on nervous system are an issue of major concern. Objective: Thus, in this study we evaluated the membrane current flow properties of Purkinje neurons after maternal exposure to 900 MHz pulsed RF-EMF. Material and methods: In this experimental study, during all days of pregnancy, rats in the EMF-exposed group were exposed to 900 MHz pulsed-EMF radiation for 6 h per day. The effects of RF-EMF exposure on the electrophysiological properties of the Purkinje cerebellum neurons from male pups were evaluated by whole- cell patch clamp recordings in current and voltage clamp modes. In voltage-clamp experiments, the holding potential was -60mV, and a depolarizing voltage step (1000 ms duration) was applied from -60 to +50 mV in 10 mV increments at 2s intervals. Results: The exposure group demonstrated reduced spontaneous firing associated with upward and rightward shift in I/V curve compared to the control rats. Moreover, the peak amplitude of the current for the exposure pups also revealed a significant decrement. The reversal potential was +40 mV and +20 mV for the control and RF-EMF groups, respectively and showed significant differences between the two groups. Conclusion: The decrease in ion's conductance could be attributed to the observed decrease in the voltage onset of the inward current, peak amplitude and voltage shift. Open access paper: ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionharm

PopulationPregnant rats; Purkinje cerebellum neurons from male pups (offspring)

Sample sizeNot supplied

ExposureRF · 900 MHz · 6 h/day during all days of pregnancy (gestation)

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Purkinje cell electrophysiological properties (whole-cell patch clamp)
Spontaneous firing rate
I/V curve shift
Peak current amplitude
Reversal potential

Limitations

Sample size not reported in the provided abstract/metadata
Exposure metric (e.g., SAR/power density) not reported in the provided abstract/metadata
Only male offspring assessed (as stated: Purkinje neurons from male pups)

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 900,
    "sar_wkg": null,
    "duration": "6 h/day during all days of pregnancy (gestation)"
  },
  "population": "Pregnant rats; Purkinje cerebellum neurons from male pups (offspring)",
  "sample_size": null,
  "outcomes": [
    "Purkinje cell electrophysiological properties (whole-cell patch clamp)",
    "Spontaneous firing rate",
    "I/V curve shift",
    "Peak current amplitude",
    "Reversal potential"
  ],
  "main_findings": "Male pups from dams exposed during gestation to 900 MHz pulsed RF-EMF (6 h/day) showed reduced spontaneous firing and an upward/rightward shift in the I/V curve versus controls. Peak current amplitude was significantly decreased, and reversal potential differed between groups (+40 mV control vs +20 mV RF-EMF).",
  "effect_direction": "harm",
  "limitations": [
    "Sample size not reported in the provided abstract/metadata",
    "Exposure metric (e.g., SAR/power density) not reported in the provided abstract/metadata",
    "Only male offspring assessed (as stated: Purkinje neurons from male pups)"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radiofrequency",
    "RF-EMF",
    "900 MHz",
    "pulsed",
    "gestation",
    "prenatal exposure",
    "rat offspring",
    "Purkinje cells",
    "cerebellum",
    "patch clamp",
    "electrophysiology"
  ],
  "suggested_hubs": [
    {
      "slug": "prenatal-exposure",
      "weight": 0.9,
      "reason": "Gestational (maternal) RF-EMF exposure with outcomes measured in offspring."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.31661/jbpe.v0i0.560
PubMed: Not supplied

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