Genomic DNA damage induced by co-exposure to DNA damaging agents and pulsed magnetic field

International journal of radiation biology · 2022 · Harm · Low evidence

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HarmLow evidencePaper 716

Pulsed magnetic field exposure (1.5 mT peak, 25 Hz) increased the level of DNA damage produced by methyl methanesulfonate (0–1%) and bleomycin (0–0.6 IU/ml) across the exposure conditions tested.

Study snapshot

PublicationInternational journal of radiation biology · 2022

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

Genomic DNA damage induced by co-exposure to DNA damaging agents and pulsed magnetic field López-Díaz B, Mercado-Sáenz S, Burgos-Molina AM, González-Vidal A, Sendra-Portero F, Ruiz-Gómez MJ. Genomic DNA damage induced by co-exposure to DNA damaging agents and pulsed magnetic field. Int J Radiat Biol. 2022 Sep 7:1-31. doi: 10.1080/09553002.2022.2121873. Abstract Purpose: Many articles describe the effects of extremely low-frequency magnetic fields (MF) on DNA damage induction. However, the mechanism of MF interaction with living matter is not yet known with certainty. Some works suggest that MF could induce an increase in the efficacy of Reactive Oxygen Species (ROS) production. This work investigates whether pulsed MF exposure produces alterations in genomic DNA damage induced by co-exposure to DNA damaging agents (bleomycin and methyl methanesulfonate (MMS)). Materials and methods: Genomic DNA, prepared from S. cerevisiae cultures, was exposed to pulsed MF (1.5 mT peak, 25 Hz) and MMS (0-1%) (15-60 minutes), and to MF and bleomycin (0-0.6 IU/ml) (24-72 hours). The damage induced to DNA was evaluated by electrophoresis and image analysis. Results: Pulsed MF induced an increment in the level of DNA damage produced by MMS and bleomycin in all groups at the exposure conditions assayed. Conclusions: Pulsed MF could modulate the cytotoxic action of MMS and bleomycin. The observed effect could be the result of a multifactorial process influenced by the type of agent that damages DNA, the dose, and the duration of the exposure to the pulsed MF. pubmed.ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

PopulationGenomic DNA prepared from Saccharomyces cerevisiae cultures

Sample sizeNot supplied

ExposureELF · 15–60 minutes (with MMS) and 24–72 hours (with bleomycin)

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Pulsed magnetic field exposure (1.5 mT peak, 25 Hz) increased the level of DNA damage produced by methyl methanesulfonate (0–1%) and bleomycin (0–0.6 IU/ml) across the exposure conditions tested.

Outcomes measured

Genomic DNA damage (electrophoresis and image analysis)

Limitations

In vitro study using genomic DNA prepared from S. cerevisiae cultures (not a human/animal in vivo study).
Co-exposure design; effects of pulsed magnetic field alone on DNA damage are not described in the abstract.
Sample size and replication details are not provided in the abstract.

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "pulsed magnetic field",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "15–60 minutes (with MMS) and 24–72 hours (with bleomycin)"
  },
  "population": "Genomic DNA prepared from Saccharomyces cerevisiae cultures",
  "sample_size": null,
  "outcomes": [
    "Genomic DNA damage (electrophoresis and image analysis)"
  ],
  "main_findings": "Pulsed magnetic field exposure (1.5 mT peak, 25 Hz) increased the level of DNA damage produced by methyl methanesulfonate (0–1%) and bleomycin (0–0.6 IU/ml) across the exposure conditions tested.",
  "effect_direction": "harm",
  "limitations": [
    "In vitro study using genomic DNA prepared from S. cerevisiae cultures (not a human/animal in vivo study).",
    "Co-exposure design; effects of pulsed magnetic field alone on DNA damage are not described in the abstract.",
    "Sample size and replication details are not provided in the abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low-frequency magnetic fields",
    "pulsed magnetic field",
    "DNA damage",
    "genomic DNA",
    "Saccharomyces cerevisiae",
    "methyl methanesulfonate",
    "MMS",
    "bleomycin",
    "reactive oxygen species",
    "ROS",
    "co-exposure"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Study investigates extremely low-frequency (25 Hz) pulsed magnetic field exposure."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/09553002.2022.2121873
PubMed: 36069754

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