Effects of 150 kHz intermediate frequency electromagnetic radiation on fertility indicators in male rats

2022 · Mixed · Low evidence

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MixedLow evidencePaper 607

In 14 male rats randomized to control vs 150 kHz EMR exposure (n=7/group) for 8 weeks, the authors report no negative effect on body mass, rectal temperature, or testicular histology. They report significant reductions in left and right testicular mass, inter…

Study snapshot

Publicationmanual · 2022
Study typeanimal
Reported directionMixed
Evidence ratingLow

Abstract

Effects of 150 kHz intermediate frequency electromagnetic radiation on fertility indicators in male rats Sundaram V, Mohammed S, Zyuzikov N. Effects of 150 kHz intermediate frequency electromagnetic radiation on fertility indicators in male rats. Heliyon. 8(12). 2022. doi: 10.1016/j.heliyon.2022.e12228. Abstract Background The present study aimed to evaluate the effects of whole-body exposure to 150 kHz Intermediate- frequency electromagnetic radiation (IF EMR) on fertility indicators of male rats since human exposure to this frequency has increased in recent years. Fourteen adult male Sprague-Dawley rats were used in this study. The rats were randomly divided into a control and an EMR group (n = 7/group). The EMR group was continuously irradiated with 150 kHz EMR for 8 weeks. Male fertility indicators, body mass, testicular mass, rectal temperature, testicular histology, histometry, sperm analysis, and serum gonadotrophic hormone levels were evaluated. Results The study showed no negative effect on body mass (grams) (323.78 ± 37.09 to 305.09 ± 26.36; p = 0.72), rectal temperature (Control: 34.5 °C–35.8 °C; EMR: 34.4 °C–36.1 °C; p < 0.05), and testicular histology. There were significant reductions in left and right testicular mass (1.04 ± 0.10 to 0.96 ± 0.32: p = 0.03 and 1.02 ± 0.08 to 0.96 ± 0.35 p = 0.04, respectively), interstitial cell count/1000 μm2 (5.33 ± 0.56 to 4.47 ± 0.48; p = 0.01), sperm motility trajectories (p = 0.05) and sperm distal cytoplasmic droplet (%) (2.27 ± 2.28 to 6.84 ± 5.01; p = 0.05). A significant increase in follicle-stimulating hormone levels was observed (13.44 ± 6.38 IU/ml to 26.96 ± 8.07 IU/ml; p = 0.01). Conclusions Most male fertility parameters of rats in the present study were not affected by 8 weeks of whole- body exposure to 150 kHz EMR. However, significant decreases in testicular mass, interstitial cell count/1000 μm2, sperm motility trajectories, and distal cytoplasmic droplets were observed, as well as an increase in FSH level. Open access paper: sciencedirect.com

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal
Effect directionmixed
PopulationAdult male Sprague-Dawley rats
Sample size14
ExposureIF · 0.15 MHz · continuous exposure for 8 weeks
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

In 14 male rats randomized to control vs 150 kHz EMR exposure (n=7/group) for 8 weeks, the authors report no negative effect on body mass, rectal temperature, or testicular histology. They report significant reductions in left and right testicular mass, interstitial cell count, sperm motility trajectories, and changes in distal cytoplasmic droplets, along with a significant increase in follicle-stimulating hormone (FSH).

Outcomes measured

  • Body mass
  • Rectal temperature
  • Testicular mass
  • Testicular histology
  • Histometry
  • Interstitial cell count
  • Sperm analysis (including motility trajectories and distal cytoplasmic droplets)
  • Serum gonadotrophic hormone levels (including FSH)

Limitations

  • Small sample size (n=7 per group).
  • Animal study; generalizability to humans is uncertain.
  • Exposure metric details (e.g., SAR) not reported in the abstract.
View raw extracted JSON
{
  "publication_year": 2022,
  "study_type": "animal",
  "exposure": {
    "band": "IF",
    "source": "whole-body exposure (experimental irradiation)",
    "frequency_mhz": 0.15,
    "sar_wkg": null,
    "duration": "continuous exposure for 8 weeks"
  },
  "population": "Adult male Sprague-Dawley rats",
  "sample_size": 14,
  "outcomes": [
    "Body mass",
    "Rectal temperature",
    "Testicular mass",
    "Testicular histology",
    "Histometry",
    "Interstitial cell count",
    "Sperm analysis (including motility trajectories and distal cytoplasmic droplets)",
    "Serum gonadotrophic hormone levels (including FSH)"
  ],
  "main_findings": "In 14 male rats randomized to control vs 150 kHz EMR exposure (n=7/group) for 8 weeks, the authors report no negative effect on body mass, rectal temperature, or testicular histology. They report significant reductions in left and right testicular mass, interstitial cell count, sperm motility trajectories, and changes in distal cytoplasmic droplets, along with a significant increase in follicle-stimulating hormone (FSH).",
  "effect_direction": "mixed",
  "limitations": [
    "Small sample size (n=7 per group).",
    "Animal study; generalizability to humans is uncertain.",
    "Exposure metric details (e.g., SAR) not reported in the abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "stance": "concern",
  "stance_confidence": 0.66,
  "summary": "This animal experiment evaluated continuous whole-body 150 kHz intermediate-frequency EMR exposure for 8 weeks in adult male Sprague-Dawley rats (n=14). The study reports no negative effects on body mass, rectal temperature, or testicular histology. However, it reports significant decreases in testicular mass, interstitial cell count, and sperm motility trajectories, plus an increase in FSH.",
  "key_points": [
    "Fourteen male rats were randomized to control or 150 kHz EMR exposure (n=7/group).",
    "Exposure was continuous whole-body irradiation for 8 weeks.",
    "No negative effects were reported for body mass, rectal temperature, or testicular histology.",
    "Significant reductions were reported in left and right testicular mass.",
    "A significant reduction in interstitial cell count per area was reported.",
    "Sperm motility trajectories and distal cytoplasmic droplet measures showed significant changes.",
    "FSH levels were reported to increase significantly in the exposed group."
  ],
  "categories": [
    "Animal Studies",
    "Intermediate Frequency (IF)",
    "Reproductive Health"
  ],
  "tags": [
    "Intermediate Frequency EMF",
    "150 kHz",
    "Male Fertility",
    "Testicular Mass",
    "Sperm Motility",
    "Follicle-Stimulating Hormone",
    "Sprague-Dawley Rats",
    "Whole-Body Exposure"
  ],
  "keywords": [
    "150 kHz",
    "intermediate frequency",
    "electromagnetic radiation",
    "male rats",
    "fertility indicators",
    "testicular mass",
    "sperm motility",
    "FSH"
  ],
  "suggested_hubs": [],
  "social": {
    "tweet": "Animal study (n=14) exposed male rats to continuous whole-body 150 kHz EMR for 8 weeks: no negative effects on body mass or testicular histology, but reported decreases in testicular mass/interstitial cells and changes in sperm motility, with increased FSH.",
    "facebook": "A small rat study examined continuous whole-body exposure to 150 kHz intermediate-frequency EMR for 8 weeks. The authors report no negative effects on body mass or testicular histology, but they observed reductions in testicular mass and interstitial cell count, changes in sperm motility measures, and increased FSH.",
    "linkedin": "Heliyon (2022) reports an animal experiment (n=14) assessing continuous whole-body 150 kHz intermediate-frequency EMR exposure for 8 weeks. Findings were mixed: no negative effects on body mass or testicular histology, but significant changes in testicular mass, interstitial cell count, sperm motility metrics, and FSH."
  }
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.heliyon.2022.e12228
PubMed: Not supplied

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