Effects of ELF-PEMF exposure on spontaneous alternation, anxiety, motor co-ordination and locomotor activity of adult wistar rats and viability of C6 (Glial) cells in culture

Toxicology · 2022 · No effect · Low evidence

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No effectLow evidencePaper 605

Study snapshot

PublicationToxicology · 2022

Study typeanimal

Reported directionNo effect

Evidence ratingLow

Abstract

Effects of ELF-PEMF exposure on spontaneous alternation, anxiety, motor co-ordination and locomotor activity of adult wistar rats and viability of C6 (Glial) cells in culture Tekam CKS, Majumdar S, Kumari P, Prajapati SK, Sahi AK, Shinde S, Singh R, Samaiya PK, Patnaik R, Krishnamurthy S, Mahto SK. Effects of ELF-PEMF exposure on spontaneous alternation, anxiety, motor co- ordination and locomotor activity of adult wistar rats and viability of C6 (Glial) cells in culture. Toxicology. 2022 Dec 24;485:153409. doi: 10.1016/j.tox.2022.153409. Abstract The effects of ELF-PEMF exposure on spontaneous alternation, anxiety, motor coordination, and locomotor activity have been discussed in various pre-clinical and clinical settings. Several epidemiological and experimental studies have demonstrated the potential effects of ELF-PEMF when exposed > ∼1 h/day; however, very few studies have focused on understanding the influence of ELF-PEMF exposure of 1-3 mT with an exposure duration of < 1 h/day on spontaneous alternation, anxiety, motor coordination, and locomotor activity. Hence, we attempted to study the effects of ELF-PEMF exposure of 1-3 mT, 50 Hz with an exposure duration of 20 min each with a 4 h gap (2 times) on the cellular proliferation and morphologies of C6 (Glial) cells and spontaneous alternation, anxiety, motor coordination and locomotor activity of Wistar rats under in vitro and in vivo conditions, respectively. The results showed that ELF-PEMF exposure did not induce any significant levels of cellular fragmentation and changes in the morphology of glial cells. Also, the outcomes revealed no noticeable effects on spontaneous alternation, anxiety, motor coordination, and locomotor activity in PEMF-exposed groups compared with the control. No undesirable side effects were observed at the highest dose (B=3 mT). We also performed histological analysis of the selected brain sections (hippocampus and cortex) following ELF-PEMF exposure. Incidentally, no significant changes were observed in cortical cell counts, tissue structure, and morphology. pubmed.ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionno_effect

PopulationAdult Wistar rats (in vivo) and C6 (glial) cells in culture (in vitro)

Sample sizeNot supplied

ExposureELF · 20 min each with a 4 h gap (2 times)

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

ELF-PEMF exposure (1–3 mT, 50 Hz; two 20-min sessions separated by 4 h) did not induce significant cellular fragmentation or morphological changes in C6 glial cells. No noticeable effects were observed on spontaneous alternation, anxiety, motor coordination, or locomotor activity in exposed rats versus controls, and histological analysis of hippocampus/cortex showed no significant changes in cortical cell counts, tissue structure, or morphology.

Outcomes measured

C6 (glial) cell proliferation
C6 (glial) cell morphology
Cellular fragmentation (glial cells)
Spontaneous alternation (rats)
Anxiety (rats)
Motor coordination (rats)
Locomotor activity (rats)
Histology of hippocampus and cortex
Cortical cell counts
Brain tissue structure and morphology

Limitations

Sample size not reported in abstract
Exposure metrics reported in mT; other dosimetry (e.g., SAR) not provided
Details of randomization/blinding and behavioral testing methods not provided in abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": "PEMF (pulsed electromagnetic field) exposure",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "20 min each with a 4 h gap (2 times)"
  },
  "population": "Adult Wistar rats (in vivo) and C6 (glial) cells in culture (in vitro)",
  "sample_size": null,
  "outcomes": [
    "C6 (glial) cell proliferation",
    "C6 (glial) cell morphology",
    "Cellular fragmentation (glial cells)",
    "Spontaneous alternation (rats)",
    "Anxiety (rats)",
    "Motor coordination (rats)",
    "Locomotor activity (rats)",
    "Histology of hippocampus and cortex",
    "Cortical cell counts",
    "Brain tissue structure and morphology"
  ],
  "main_findings": "ELF-PEMF exposure (1–3 mT, 50 Hz; two 20-min sessions separated by 4 h) did not induce significant cellular fragmentation or morphological changes in C6 glial cells. No noticeable effects were observed on spontaneous alternation, anxiety, motor coordination, or locomotor activity in exposed rats versus controls, and histological analysis of hippocampus/cortex showed no significant changes in cortical cell counts, tissue structure, or morphology.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure metrics reported in mT; other dosimetry (e.g., SAR) not provided",
    "Details of randomization/blinding and behavioral testing methods not provided in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-PEMF",
    "pulsed electromagnetic field",
    "50 Hz",
    "1-3 mT",
    "Wistar rats",
    "C6 glial cells",
    "anxiety",
    "locomotor activity",
    "motor coordination",
    "spontaneous alternation",
    "histology",
    "hippocampus",
    "cortex"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Study evaluates extremely low frequency (50 Hz) PEMF exposure effects in vivo and in vitro."
    },
    {
      "slug": "animal-studies",
      "weight": 0.85,
      "reason": "Includes behavioral and histological outcomes in adult Wistar rats."
    },
    {
      "slug": "in-vitro",
      "weight": 0.7,
      "reason": "Assesses viability/morphology outcomes in cultured C6 glial cells."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.tox.2022.153409
PubMed: 36572170

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