Assessment of protein and DNA polymorphisms in corn (Zea mays) under the effect of non-ionizing electromagnetic radiation

2023 · Harm · Low evidence

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HarmLow evidencePaper 482

Study snapshot

Publicationmanual · 2023

Study typeanimal

Reported directionHarm

Evidence ratingLow

Abstract

Assessment of protein and DNA polymorphisms in corn (Zea mays) under the effect of non-ionizing electromagnetic radiation Abdelhaliem E, Abdalla HM, Bolbol AA, Shehata RS. Assessment of protein and DNA polymorphisms in corn (Zea mays) under the effect of non-ionizing electromagnetic radiation. Caryologia [Internet]. 2023. 75(4). Abstract Many reports highlight biological responses of crop plants after non-ionizing electromagnetic radiation (EMR) exposure based on the phenotypic and physiological levels. So, this study aimed to estimate genetic alterations in proteins, isozymes, and DNA banding patterns as well as the extent of nuclear DNA damage of economic corn (Zea mays) under the stress of EMR using accurate and reliable bioassays like sodium dodecyl sulfate- polyacrylamide gel electrophoresis (SDS-PAGE), isozymes (Leucine- aminopeptidase, Esterases, Peroxidase, and Catalases), random amplified polymorphic DNA- polymerase chain reaction (RAPD-PCR), and Comet Assay, respectively. SDS-PAGE analysis showed distinct polymorphisms (96.66%) between EMR exposed and non- exposed corn seedlings depending on the number and type of bands, their intensities as well as molecular weight which ranged from (60.27 to 192.35 kDa), gain, and loss of bands. The four isozymes generated varies isozymatic polymorphisms based on relative front, zymogram number, and optical intensities. RAPD analysis generated 85 amplified DNA products with high polymorphism values ranged from 90.91 to 100% based on primers, band type, DNA sizes which ranged from 153 to 1008-bp, lose, gain, and intensity of DNA bands. Comet Assay scored highest extent of loosed DNA from nuclei (DNA damage) reached the value of (tailed ratio 20%) at EMR exposed corn nuclei for 5 days compared to non-exposed nuclei which reached the value of (tailed ratio 3%). This study concluded that each EMR exposure time had unique interaction with proteins, isozymes, and DNA of corn cells exhibiting wide range of genotoxic stress and subsequently, adversely effect on growth and yield of this sensitive crop plants. Open access paper: riviste.fupress.net

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionharm

PopulationCorn (Zea mays) seedlings

Sample sizeNot supplied

ExposureExposure for up to 5 days (time-dependent effects mentioned; specific schedule not fully described)

Evidence strengthLowConfidence: 74% · Peer reviewed: unknown

Main findings

EMR-exposed corn seedlings showed distinct protein polymorphisms by SDS-PAGE (reported 96.66%) and isozyme pattern differences across four enzyme systems. RAPD-PCR produced 85 DNA products with high polymorphism (90.91–100% depending on primer), and comet assay indicated higher nuclear DNA damage in exposed nuclei (tailed ratio 20% at 5 days) versus non-exposed (tailed ratio 3%).

Outcomes measured

Protein banding polymorphisms (SDS-PAGE)
Isozyme polymorphisms (Leucine-aminopeptidase, Esterases, Peroxidase, Catalases)
DNA banding polymorphisms (RAPD-PCR)
Nuclear DNA damage (Comet assay)
Growth and yield (mentioned as adversely affected in conclusion; not quantified in abstract)

Limitations

EMR exposure characteristics (frequency, field strength/power density, modulation, source) not reported in the abstract
Sample size and replication not reported in the abstract
Quantitative growth/yield results not provided in the abstract (only stated in conclusion)
Unclear exposure regimen details beyond mention of exposure time and a 5-day comparison

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": null,
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "Exposure for up to 5 days (time-dependent effects mentioned; specific schedule not fully described)"
  },
  "population": "Corn (Zea mays) seedlings",
  "sample_size": null,
  "outcomes": [
    "Protein banding polymorphisms (SDS-PAGE)",
    "Isozyme polymorphisms (Leucine-aminopeptidase, Esterases, Peroxidase, Catalases)",
    "DNA banding polymorphisms (RAPD-PCR)",
    "Nuclear DNA damage (Comet assay)",
    "Growth and yield (mentioned as adversely affected in conclusion; not quantified in abstract)"
  ],
  "main_findings": "EMR-exposed corn seedlings showed distinct protein polymorphisms by SDS-PAGE (reported 96.66%) and isozyme pattern differences across four enzyme systems. RAPD-PCR produced 85 DNA products with high polymorphism (90.91–100% depending on primer), and comet assay indicated higher nuclear DNA damage in exposed nuclei (tailed ratio 20% at 5 days) versus non-exposed (tailed ratio 3%).",
  "effect_direction": "harm",
  "limitations": [
    "EMR exposure characteristics (frequency, field strength/power density, modulation, source) not reported in the abstract",
    "Sample size and replication not reported in the abstract",
    "Quantitative growth/yield results not provided in the abstract (only stated in conclusion)",
    "Unclear exposure regimen details beyond mention of exposure time and a 5-day comparison"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "unknown",
  "keywords": [
    "non-ionizing electromagnetic radiation",
    "EMR",
    "Zea mays",
    "corn",
    "genotoxicity",
    "DNA damage",
    "comet assay",
    "RAPD-PCR",
    "SDS-PAGE",
    "isozymes",
    "polymorphism"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: Not supplied

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