Streptosporangium minutum sp. nov., isolated from garden soil exposed to microwave radiation.
← Research streamStrain M26 was isolated from garden soil that had been pre-treated with microwave radiation and was characterized via polyphasic taxonomy as a new species, proposed as Streptosporangium minutum. The strain exhibited antibiosis against a range of pathogenic ba…
Study snapshot
Abstract
The actinobacterium, strain M26, was isolated from garden soil that was pre-treated with microwave radiation. The soil sample was collected in Roodepoort, Gauteng Province, South Africa as part of an antibiotic-screening programme. The isolate produced branched vegetative mycelium with sporangiophores bearing small sporangia ranging from 3 to 6 μm in diameter. Rapid genus identification revealed that the isolate belongs to the genus Streptosporangium. To confirm this result, the strain was subjected to polyphasic taxonomic characterisation. Chemotaxonomic characteristics were as follows: meso-DAP in the peptidoglycan, the whole-cell hydrolysate yielded madurose, predominant menaquinones were MK9 (21%), MK9(H) (40%), MK9(H) (31%) and MK9(H) (3%); the polar lipid profile included an aminolipid, phosphoglycolipids, phosphatidylethanolamine, and phosphatidylmonomethylethanolamine. In addition, the fatty acid profile showed the presence of C (12.8%), Cω8c (14.2%), and 10-methyl-C (15.8%). Furthermore, 16S rRNA gene sequence phylogenetic analysis showed that the strain is closely related to members of the genus Streptosporangium, which supports its classification within the family Streptosporangiaceae. Strain M26 exhibited antibiosis against a range of pathogenic bacteria, including, but not limited to Acinetobacter baumannii ATCC 19606, Enterobacter cloacae subsp. cloacae ATCC BAA-1143, Enterococcus faecalis ATCC 51299 (vancomycin resistant), Escherichia coli ATCC 25922, Listeria monocytogenes ATCC 19111, Mycobacterium tuberculosis H37Rv, Pseudomonas aeruginosa ATCC 27853, Salmonella enterica subsp. arizonae ATCC 13314, and the methicillin-resistant Staphylococcus aureus subsp. aureus ATCC 33591 (MRSA). The name Streptosporangium minutum is proposed with the type strain M26 (=LMG 28850 =NRRL B-65295).
AI evidence extraction
Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.
Main findings
Strain M26 was isolated from garden soil that had been pre-treated with microwave radiation and was characterized via polyphasic taxonomy as a new species, proposed as Streptosporangium minutum. The strain exhibited antibiosis against a range of pathogenic bacteria including MRSA, vancomycin-resistant Enterococcus faecalis, and Mycobacterium tuberculosis H37Rv.
Outcomes measured
- Isolation and taxonomic characterization of a novel actinobacterium strain (M26)
- Antibiosis/antibacterial activity against multiple pathogenic bacteria
Limitations
- Microwave exposure parameters (e.g., frequency, power, duration) are not reported in the abstract.
- No comparison is described between microwave-treated and untreated soil for isolation yield or microbial changes.
- Not a health-effects study; outcomes relate to microbial isolation/taxonomy and antibacterial activity.
View raw extracted JSON
{
"study_type": "other",
"exposure": {
"band": "microwave",
"source": "soil pre-treatment",
"frequency_mhz": null,
"sar_wkg": null,
"duration": null
},
"population": null,
"sample_size": null,
"outcomes": [
"Isolation and taxonomic characterization of a novel actinobacterium strain (M26)",
"Antibiosis/antibacterial activity against multiple pathogenic bacteria"
],
"main_findings": "Strain M26 was isolated from garden soil that had been pre-treated with microwave radiation and was characterized via polyphasic taxonomy as a new species, proposed as Streptosporangium minutum. The strain exhibited antibiosis against a range of pathogenic bacteria including MRSA, vancomycin-resistant Enterococcus faecalis, and Mycobacterium tuberculosis H37Rv.",
"effect_direction": "unclear",
"limitations": [
"Microwave exposure parameters (e.g., frequency, power, duration) are not reported in the abstract.",
"No comparison is described between microwave-treated and untreated soil for isolation yield or microbial changes.",
"Not a health-effects study; outcomes relate to microbial isolation/taxonomy and antibacterial activity."
],
"evidence_strength": "insufficient",
"confidence": 0.74,
"peer_reviewed_likely": "yes",
"keywords": [
"microwave radiation",
"soil pre-treatment",
"actinobacterium",
"Streptosporangium",
"novel species",
"polyphasic taxonomy",
"16S rRNA",
"antibiosis",
"antibiotic screening"
],
"suggested_hubs": []
}AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.
Identifiers and source
DOI: 10.1038/s41429-018-0036-0
PubMed: 29515231