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[Inhibitory effect of microwave radiation on proliferation of human pancreatic cancer JF305 cells and its mechanism].

Wei sheng yan jiu = Journal of hygiene research · 2013 · Benefit · Low evidence

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BenefitLow evidencePaper 6536

Exposure to 2450 MHz microwave radiation (2.5–20.0 mW/cm2 for 20 min) was associated with significantly higher proliferation inhibition rates versus control in JF305 cells. Microwave exposure induced apoptosis and increased caspase-3 activity and caspase-3 an…

Study snapshot

PublicationWei sheng yan jiu = Journal of hygiene research · 2013
Study typein_vitro
Reported directionBenefit
Evidence ratingLow

Abstract

OBJECTIVE: To investigate on the proliferation effect of different intensities 2450 MHz microwave radiation on human pancreatic cancer JF305 cells and its possible mechanism. METHODS: JF305 cells were radiated by intensity of 2.5, 5.0, 10.0, 15.0 and 20.0 mW/cm2 microwave for 20 min. The proliferation capacity of JF305 was measured by MTT assays, Annexin V-FITC and PI staining was used for detecting cell apoptosis. The activity of Caspase-3 was examined. The expressions of Caspase-3 and HSP 70 protein after the cell treatment with microwave were detected by Western blotting. RESULTS: After microwave radiation, the proliferation inhibition rates of JF305 cells were significantly higher compared with control group. Annexin V-FITC and PI staining result showed that microwave radiation could induce cell apoptosis. Caspase-3 increased after radiated by microwave, compared with control group (P < 0.05). Results of Western blotting showed that the expression of Caspase-3 and HSP 70 protein increased significantly in different dosage radiation group. CONCLUSION: Microwave radiation can inhibit the proliferation of JF305 cells, the possible mechanism may be related with inducing cell apoptosis by changing of stress level.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionbenefit
PopulationHuman pancreatic cancer JF305 cells
Sample sizeNot supplied
Exposuremicrowave · 2450 MHz · 20 min
Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Exposure to 2450 MHz microwave radiation (2.5–20.0 mW/cm2 for 20 min) was associated with significantly higher proliferation inhibition rates versus control in JF305 cells. Microwave exposure induced apoptosis and increased caspase-3 activity and caspase-3 and HSP70 protein expression compared with control.

Outcomes measured

  • Cell proliferation (MTT assay)
  • Apoptosis (Annexin V-FITC/PI staining)
  • Caspase-3 activity
  • Caspase-3 protein expression (Western blot)
  • HSP70 protein expression (Western blot)

Limitations

  • In vitro study in a single human cancer cell line (JF305)
  • No sample size or replication details provided in the abstract
  • Exposure metric reported as power density (mW/cm2) without SAR or temperature control details
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": 2450,
    "sar_wkg": null,
    "duration": "20 min"
  },
  "population": "Human pancreatic cancer JF305 cells",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation (MTT assay)",
    "Apoptosis (Annexin V-FITC/PI staining)",
    "Caspase-3 activity",
    "Caspase-3 protein expression (Western blot)",
    "HSP70 protein expression (Western blot)"
  ],
  "main_findings": "Exposure to 2450 MHz microwave radiation (2.5–20.0 mW/cm2 for 20 min) was associated with significantly higher proliferation inhibition rates versus control in JF305 cells. Microwave exposure induced apoptosis and increased caspase-3 activity and caspase-3 and HSP70 protein expression compared with control.",
  "effect_direction": "benefit",
  "limitations": [
    "In vitro study in a single human cancer cell line (JF305)",
    "No sample size or replication details provided in the abstract",
    "Exposure metric reported as power density (mW/cm2) without SAR or temperature control details"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "2450 MHz",
    "microwave radiation",
    "power density",
    "MTT",
    "apoptosis",
    "Annexin V-FITC",
    "PI staining",
    "caspase-3",
    "HSP70",
    "pancreatic cancer",
    "JF305 cells"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 24459920

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