Rapid de-O-glycosylation concomitant with peptide labeling using microwave radiation and an alkyl amine base.

Analytical chemistry · 2010 · Unclear · Insufficient evidence

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UnclearInsufficient evidencePaper 6514

A mild base (dimethylamine) with microwave radiation accelerated and improved the efficiency of O-linked glycan release, with concurrent incorporation of dimethylamine into former O-linked sites. The protocol was established using a synthetic O-GlcNAc peptide…

Study snapshot

PublicationAnalytical chemistry · 2010

Study typeother

Reported directionUnclear

Evidence ratingInsufficient

Abstract

Procedures are detailed for a quantitative release of O-linked glycans from peptides that now provide a shorter reaction time, a possible identification of O-linked sites, and a quantification of all reaction products. The release was initiated by a mild base, dimethylamine, and accelerated by microwave radiation. Differential analysis using standard glycoproteins has shown improved release efficiency concurrent with facile incorporation of dimethylamine into the former O-linked sites. In situ glycan reduction insures protection against peeling and is synchronous with subsequent studies by high performance MS(n) sequencing. The protocols were established with a synthetic O-GlcNAc peptide that would mimic the linkage chemistry and applied to a well characterized glycoprotein bovine fetuin with both N- and O-linked glycans and a highly glycosylated swine mucin.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeother

Effect directionunclear

PopulationNot supplied

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthInsufficientConfidence: 66% · Peer reviewed: yes

Main findings

Outcomes measured

Efficiency of O-linked glycan release from peptides/glycoproteins
Incorporation of dimethylamine into former O-linked sites (site labeling)
Quantification of reaction products
Protection against peeling via in situ glycan reduction
Compatibility with high performance MS(n) sequencing

View raw extracted JSON

{
  "study_type": "other",
  "exposure": {
    "band": null,
    "source": "microwave radiation (laboratory/analytical protocol)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Efficiency of O-linked glycan release from peptides/glycoproteins",
    "Incorporation of dimethylamine into former O-linked sites (site labeling)",
    "Quantification of reaction products",
    "Protection against peeling via in situ glycan reduction",
    "Compatibility with high performance MS(n) sequencing"
  ],
  "main_findings": "A mild base (dimethylamine) with microwave radiation accelerated and improved the efficiency of O-linked glycan release, with concurrent incorporation of dimethylamine into former O-linked sites. The protocol was established using a synthetic O-GlcNAc peptide and applied to bovine fetuin and swine mucin.",
  "effect_direction": "unclear",
  "limitations": [],
  "evidence_strength": "insufficient",
  "confidence": 0.66,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "O-glycosylation",
    "de-O-glycosylation",
    "dimethylamine",
    "O-linked glycans",
    "O-GlcNAc peptide",
    "bovine fetuin",
    "swine mucin",
    "mass spectrometry",
    "MS(n) sequencing"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1021/ac902734w
PubMed: 20178317

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