Effect of microwave radiation, pasteurization and lyophilization on the ability of human milk to inhibit Escherichia coli adherence to HEp-2 cells.

Journal of diarrhoeal diseases research · 1996 · No effect · Low evidence

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No effectLow evidencePaper 6333

Study snapshot

PublicationJournal of diarrhoeal diseases research · 1996

Study typein_vitro

Reported directionNo effect

Evidence ratingLow

Abstract

The effect of different physical treatments on the ability of colostrum and human milk to inhibit the adherence of enteropathogenic Escherichia coli (EPEC) to human epithelial cells was studied. Pools of colostrum and milk were submitted to microwave radiation, pasteurization or lyophilization, and then tested for the ability to inhibit the adherence of EPEC O111:H- to HEp-2 cells. The inhibitory effect of untreated colostrum and human milk on localized adherence was not significantly modified after exposure to any treatment. The total protein values of colostrum and milk were maintained, but IgA concentration and colostral anti-EPEC IgA were reduced after pasteurization. Nevertheless, the remaining IgA was sufficient to be effective in adhesion inhibition assay. Western blotting assays carried out with EPEC antigens showed that the treated and untreated pools recognize a 94-kDa outer-membrane protein which molecular weight is compatible with intimin, an EPEC adhesin related to bacterial attachment to epithelial cells. These results suggest that the protection of colostrum and milk to infantile diarrhoea due to EPEC remains unalterable after the physical treatments studied.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionno_effect

PopulationNot supplied

Sample sizeNot supplied

Exposuremicrowave

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Pools of colostrum and human milk exposed to microwave radiation, pasteurization, or lyophilization did not show a significant change in their ability to inhibit localized adherence of EPEC O111:H- to HEp-2 cells compared with untreated samples. Total protein values were maintained; IgA concentration and colostral anti-EPEC IgA were reduced after pasteurization, but remaining IgA was still sufficient for the adhesion inhibition assay.

Outcomes measured

Inhibition of enteropathogenic Escherichia coli (EPEC) adherence to HEp-2 cells
Total protein values in colostrum and human milk
IgA concentration
Colostral anti-EPEC IgA
Recognition of EPEC antigens (94-kDa outer-membrane protein compatible with intimin) by Western blot

Limitations

Exposure parameters for microwave radiation (e.g., frequency, power, duration) not reported in the abstract
Sample size and number of pools/replicates not reported
In vitro assay (HEp-2 cells) may not reflect in vivo infant outcomes

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "microwave",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Inhibition of enteropathogenic Escherichia coli (EPEC) adherence to HEp-2 cells",
    "Total protein values in colostrum and human milk",
    "IgA concentration",
    "Colostral anti-EPEC IgA",
    "Recognition of EPEC antigens (94-kDa outer-membrane protein compatible with intimin) by Western blot"
  ],
  "main_findings": "Pools of colostrum and human milk exposed to microwave radiation, pasteurization, or lyophilization did not show a significant change in their ability to inhibit localized adherence of EPEC O111:H- to HEp-2 cells compared with untreated samples. Total protein values were maintained; IgA concentration and colostral anti-EPEC IgA were reduced after pasteurization, but remaining IgA was still sufficient for the adhesion inhibition assay.",
  "effect_direction": "no_effect",
  "limitations": [
    "Exposure parameters for microwave radiation (e.g., frequency, power, duration) not reported in the abstract",
    "Sample size and number of pools/replicates not reported",
    "In vitro assay (HEp-2 cells) may not reflect in vivo infant outcomes"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "microwave radiation",
    "pasteurization",
    "lyophilization",
    "human milk",
    "colostrum",
    "Escherichia coli",
    "EPEC",
    "HEp-2 cells",
    "IgA",
    "intimin",
    "adhesion inhibition"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 8870401

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