Magnetofection of miR-21 promoted by electromagnetic field and iron oxide nanoparticles via the p38 MAPK pathway contributes to osteogenesis and angiogenesis for intervertebral fusion.

Journal of nanobiotechnology · 2023 · Benefit · Low evidence

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BenefitLow evidencePaper 5808

Co-stimulation with electromagnetic field (EMF) and iron oxide nanoparticles (IONPs) significantly enhanced magnetofection efficiency. Magnetofection of miR-21 into BMSCs and HUVECs promoted osteogenesis and angiogenesis in vitro and in vivo, and the enhanced…

Study snapshot

PublicationJournal of nanobiotechnology · 2023

Study typeanimal

Reported directionBenefit

Evidence ratingLow

Abstract

BACKGROUND: Magnetofection-mediated gene delivery shows great therapeutic potential through the regulation of the direction and degree of differentiation. Lumbar degenerative disc disease (DDD) is a serious global orthopaedic problem. However, even though intervertebral fusion is the gold standard for the treatment of DDD, its therapeutic effect is unsatisfactory. Here, we described a novel magnetofection system for delivering therapeutic miRNAs to promote osteogenesis and angiogenesis in patients with lumbar DDD. RESULTS: Co-stimulation with electromagnetic field (EMF) and iron oxide nanoparticles (IONPs) enhanced magnetofection efficiency significantly. Moreover, in vitro, magnetofection of miR-21 into bone marrow mesenchymal stem cells (BMSCs) and human umbilical endothelial cells (HUVECs) influenced their cellular behaviour and promoted osteogenesis and angiogenesis. Then, gene-edited seed cells were planted onto polycaprolactone (PCL) and hydroxyapatite (HA) scaffolds (PCL/HA scaffolds) and evolved into the ideal tissue-engineered bone to promote intervertebral fusion. Finally, our results showed that EMF and polyethyleneimine (PEI)@IONPs were enhancing transfection efficiency by activating the p38 MAPK pathway. CONCLUSION: Our findings illustrate that a magnetofection system for delivering miR-21 into BMSCs and HUVECs promoted osteogenesis and angiogenesis in vitro and in vivo and that magnetofection transfection efficiency improved significantly under the co-stimulation of EMF and IONPs. Moreover, it relied on the activation of p38 MAPK pathway. This magnetofection system could be a promising therapeutic approach for various orthopaedic diseases.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionbenefit

PopulationNot supplied

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthLowConfidence: 66% · Peer reviewed: yes

Main findings

Outcomes measured

Magnetofection/transfection efficiency
Osteogenesis
Angiogenesis
Intervertebral fusion (tissue-engineered bone)
p38 MAPK pathway activation

Limitations

EMF exposure parameters (e.g., frequency, intensity, duration) not reported in abstract
Sample size not reported in abstract
In vivo model details not reported in abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": null,
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Magnetofection/transfection efficiency",
    "Osteogenesis",
    "Angiogenesis",
    "Intervertebral fusion (tissue-engineered bone)",
    "p38 MAPK pathway activation"
  ],
  "main_findings": "Co-stimulation with electromagnetic field (EMF) and iron oxide nanoparticles (IONPs) significantly enhanced magnetofection efficiency. Magnetofection of miR-21 into BMSCs and HUVECs promoted osteogenesis and angiogenesis in vitro and in vivo, and the enhanced transfection efficiency under EMF and PEI@IONPs was associated with activation of the p38 MAPK pathway.",
  "effect_direction": "benefit",
  "limitations": [
    "EMF exposure parameters (e.g., frequency, intensity, duration) not reported in abstract",
    "Sample size not reported in abstract",
    "In vivo model details not reported in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.66,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "magnetofection",
    "electromagnetic field",
    "iron oxide nanoparticles",
    "IONPs",
    "PEI@IONPs",
    "miR-21",
    "BMSCs",
    "HUVECs",
    "osteogenesis",
    "angiogenesis",
    "intervertebral fusion",
    "p38 MAPK"
  ],
  "suggested_hubs": [
    {
      "slug": "engineering",
      "weight": 0.78,
      "reason": "Study develops an EMF+IONP magnetofection system to enhance gene delivery and tissue-engineered bone formation."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1186/s12951-023-01789-3
PubMed: 36694219

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