Effects of pulsed electromagnetic fields and N-acetylcysteine on transplantation of vitrified mouse ovarian tissue.

Electromagnetic biology and medicine · 2023 · Benefit · Low evidence

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BenefitLow evidencePaper 5782

Study snapshot

PublicationElectromagnetic biology and medicine · 2023

Study typeanimal

Reported directionBenefit

Evidence ratingLow

Abstract

In this experimental study, adult female NMRI mice were randomly assigned to five groups: control ;(fresh ovarian transplantation, OT); sham ;(vitrified OT); NAC ;(vitrified OT treated with N-acetyl cysteine, NAC); EMF ;(vitrified OT treated with pulsed electromagnetic fields, PEMF); and NAC+EMF ;(vitrified OT combined with NAC and PEMF). We conducted histological assessments to evaluate follicle reservation and vascularization. Furthermore, we examined the relative expression of , and genes on days 2 and 7 after OT. Additionally, we measured total antioxidant capacity (TAC), malondialdehyde (MDA) levels, as well as the activity of superoxide dismutase (SOD) and glutathione peroxidase (GPX). Our results demonstrated that NAC, PEMF, and NAC+PEMF treatments significantly increased the number of follicles. Moreover, we observed a more pronounced development of vascularization in the NAC, PEMF, and PEMF+NAC groups. The relative expression levels of , and were significantly elevated in the NAC, PEMF, and NAC+PEMF groups. Notably, TAC levels decreased significantly in the NAC group compared to the control group. Additionally, the MDA level showed a significant decrease in the PEMF+NAC group when compared to the other groups. Overall, the combination of NAC and PEMF exhibited a synergistic effect in promoting angiogenesis and protecting against oxidative stress and inflammation during OT.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeanimal

Effect directionbenefit

PopulationAdult female NMRI mice (ovarian tissue transplantation model; fresh vs vitrified ovarian transplantation)

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In vitrified ovarian transplantation, NAC, PEMF, and NAC+PEMF groups showed significantly increased follicle numbers and more pronounced vascularization compared with sham vitrified OT. Relative expression levels of unspecified genes were significantly elevated in NAC, PEMF, and NAC+PEMF groups; TAC decreased in the NAC group vs control, and MDA decreased in the PEMF+NAC group vs other groups. The authors conclude NAC+PEMF had a synergistic effect promoting angiogenesis and protection against oxidative stress/inflammation during ovarian transplantation.

Outcomes measured

Follicle number/reservation (histology)
Vascularization/angiogenesis (histology)
Gene expression on days 2 and 7 after ovarian transplantation (genes not specified in abstract)
Total antioxidant capacity (TAC)
Malondialdehyde (MDA)
Superoxide dismutase (SOD) activity
Glutathione peroxidase (GPX) activity
Oxidative stress and inflammation markers (as described)

Limitations

Frequency/intensity/dose and exposure duration for PEMF not reported in abstract
Sample size not provided in abstract
Specific genes assessed are not named in the abstract
Animal model; generalizability to humans not addressed in abstract

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": null,
    "source": "pulsed electromagnetic fields (PEMF)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Adult female NMRI mice (ovarian tissue transplantation model; fresh vs vitrified ovarian transplantation)",
  "sample_size": null,
  "outcomes": [
    "Follicle number/reservation (histology)",
    "Vascularization/angiogenesis (histology)",
    "Gene expression on days 2 and 7 after ovarian transplantation (genes not specified in abstract)",
    "Total antioxidant capacity (TAC)",
    "Malondialdehyde (MDA)",
    "Superoxide dismutase (SOD) activity",
    "Glutathione peroxidase (GPX) activity",
    "Oxidative stress and inflammation markers (as described)"
  ],
  "main_findings": "In vitrified ovarian transplantation, NAC, PEMF, and NAC+PEMF groups showed significantly increased follicle numbers and more pronounced vascularization compared with sham vitrified OT. Relative expression levels of unspecified genes were significantly elevated in NAC, PEMF, and NAC+PEMF groups; TAC decreased in the NAC group vs control, and MDA decreased in the PEMF+NAC group vs other groups. The authors conclude NAC+PEMF had a synergistic effect promoting angiogenesis and protection against oxidative stress/inflammation during ovarian transplantation.",
  "effect_direction": "benefit",
  "limitations": [
    "Frequency/intensity/dose and exposure duration for PEMF not reported in abstract",
    "Sample size not provided in abstract",
    "Specific genes assessed are not named in the abstract",
    "Animal model; generalizability to humans not addressed in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "pulsed electromagnetic fields",
    "PEMF",
    "N-acetylcysteine",
    "NAC",
    "vitrification",
    "ovarian tissue transplantation",
    "mouse",
    "angiogenesis",
    "vascularization",
    "oxidative stress",
    "malondialdehyde",
    "total antioxidant capacity",
    "SOD",
    "GPX"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/15368378.2023.2246503
PubMed: 37573526

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