RF Safe

Osteoinductivity of polycaprolactone nanofibers grafted functionalized with carboxymethyl chitosan: Synergic effect of β-carotene and electromagnetic field.

International journal of biological macromolecules · 2020 · Benefit · Low evidence

← Research stream
BenefitLow evidencePaper 5654

CMC-grafted PCL scaffolds showed osteoinductive effects, with mineralization (nodal calcium) observed after 14 days with or without external stimulation. Osteodifferentiation of ADSCs was reported as promoted by external stimulation factors including β-carote…

Study snapshot

PublicationInternational journal of biological macromolecules · 2020
Study typein_vitro
Reported directionBenefit
Evidence ratingLow

Abstract

In this study, carboxymethyl chitosan (CMC) was grafted on polycaprolactone (PCL) nanofibers to fabricate scaffolds for bone tissue engineering. The electrospun PCL nanofibers were treated by cold atmospheric plasma (CAP) of helium to generate the reactive functions necessary for CMC grafting. β-carotene (βC) as a biochemical clue and electromagnetic field (EMF, 31.4 μT, 1 h per day) as a biophysical stimulator were used to promote the proliferation and osteodifferentiation of adipose mesenchymal stem cells (ADSCs). Alizarin red staining and calcium content results indicated the generation of nodal calcium on the CMC30%-g-PCL scaffold after 14 days of incubation in the presence or absence of external stimulation factors. Immunocytochemistry (ICC) results confirmed the expression of osteonectin protein for the stem cells seeded on CMC30%-g-PCL with or without using βC or EMF. These results suggest that the fabricated CMC-grafted scaffolds have the ability to self-differentiate stem cells to osteoblasts due to the osteoinductive effects of the grafted CMC. Furthermore, the osteodifferentiation of ADSCs is promoted by using an external stimulation factor such as βC or EMF.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionbenefit
PopulationAdipose mesenchymal stem cells (ADSCs) cultured on carboxymethyl chitosan (CMC)-grafted polycaprolactone (PCL) nanofiber scaffolds
Sample sizeNot supplied
ExposureELF · 1 h per day
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

CMC-grafted PCL scaffolds showed osteoinductive effects, with mineralization (nodal calcium) observed after 14 days with or without external stimulation. Osteodifferentiation of ADSCs was reported as promoted by external stimulation factors including β-carotene or an electromagnetic field (31.4 μT, applied 1 h/day).

Outcomes measured

  • ADSC proliferation
  • Osteodifferentiation/osteogenic differentiation
  • Mineralization (Alizarin red staining)
  • Calcium content
  • Osteonectin protein expression (immunocytochemistry)

Limitations

  • Sample size not reported in abstract
  • EMF frequency and waveform not reported in abstract
  • In vitro study; findings may not generalize to in vivo or human health outcomes
  • Exposure details beyond field strength and daily duration not provided
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "1 h per day"
  },
  "population": "Adipose mesenchymal stem cells (ADSCs) cultured on carboxymethyl chitosan (CMC)-grafted polycaprolactone (PCL) nanofiber scaffolds",
  "sample_size": null,
  "outcomes": [
    "ADSC proliferation",
    "Osteodifferentiation/osteogenic differentiation",
    "Mineralization (Alizarin red staining)",
    "Calcium content",
    "Osteonectin protein expression (immunocytochemistry)"
  ],
  "main_findings": "CMC-grafted PCL scaffolds showed osteoinductive effects, with mineralization (nodal calcium) observed after 14 days with or without external stimulation. Osteodifferentiation of ADSCs was reported as promoted by external stimulation factors including β-carotene or an electromagnetic field (31.4 μT, applied 1 h/day).",
  "effect_direction": "benefit",
  "limitations": [
    "Sample size not reported in abstract",
    "EMF frequency and waveform not reported in abstract",
    "In vitro study; findings may not generalize to in vivo or human health outcomes",
    "Exposure details beyond field strength and daily duration not provided"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "polycaprolactone",
    "nanofibers",
    "carboxymethyl chitosan",
    "cold atmospheric plasma",
    "beta-carotene",
    "electromagnetic field",
    "31.4 μT",
    "adipose mesenchymal stem cells",
    "osteodifferentiation",
    "bone tissue engineering"
  ],
  "suggested_hubs": []
}
AI can be wrong. Always verify against the paper.

AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.ijbiomac.2020.02.036
PubMed: 32035957

Read the original paper →

Related papers

← Back to the Research Library