Non-Ionizing Radiation for Cardiac Human Amniotic Mesenchymal Stromal Cell Commitment: A Physical Strategy in Regenerative Medicine.

International journal of molecular sciences · 2018 · Mixed · Low evidence

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MixedLow evidencePaper 5518

Study snapshot

PublicationInternational journal of molecular sciences · 2018

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

Cell therapy is an innovative strategy for tissue repair, since adult stem cells could have limited regenerative ability as in the case of myocardial damage. This leads to a local contractile dysfunction due to scar formation. For these reasons, refining strategy approaches for "in vitro" stem cell commitment, preparatory to the "in vivo" stem cell differentiation, is imperative. In this work, we isolated and characterized at molecular and cellular level, human Amniotic Mesenchymal Stromal Cells (hAMSCs) and exposed them to a physical Extremely Low Frequency Electromagnetic Field (ELF-EMF) stimulus and to a chemical Nitric Oxide treatment. Physically exposed cells showed a decrease of cell proliferation and no change in metabolic activity, cell vitality and apoptotic rate. An increase in the mRNA expression of cardiac and angiogenic differentiation markers, confirmed at the translational level, was also highlighted in exposed cells. Our data, for the first time, provide evidence that physical ELF-EMF stimulus (7 Hz, 2.5 µT), similarly to the chemical treatment, is able to trigger hAMSC cardiac commitment. More importantly, we also observed that only the physical stimulus is able to induce both types of commitments contemporarily (cardiac and angiogenic), suggesting its potential use to obtain a better regenerative response in cell-therapy protocols.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman amniotic mesenchymal stromal cells (hAMSCs)

Sample sizeNot supplied

ExposureELF · 0.007 MHz

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

hAMSCs exposed to an ELF-EMF stimulus (7 Hz, 2.5 µT) showed decreased cell proliferation with no change in metabolic activity, vitality, or apoptotic rate. Exposed cells showed increased mRNA expression of cardiac and angiogenic differentiation markers, confirmed at the translational level, and the authors report ELF-EMF exposure could trigger cardiac commitment and induce both cardiac and angiogenic commitments contemporaneously.

Outcomes measured

Cell proliferation
Metabolic activity
Cell vitality
Apoptotic rate
mRNA expression of cardiac differentiation markers
mRNA expression of angiogenic differentiation markers
Protein (translational) confirmation of differentiation markers
Cardiac commitment
Angiogenic commitment

Limitations

In vitro study; findings may not translate to in vivo or clinical outcomes
Exposure duration not reported in the abstract
Sample size not reported in the abstract
Details of exposure setup and dosimetry beyond frequency and magnetic flux density not reported in the abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": 0.007,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human amniotic mesenchymal stromal cells (hAMSCs)",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation",
    "Metabolic activity",
    "Cell vitality",
    "Apoptotic rate",
    "mRNA expression of cardiac differentiation markers",
    "mRNA expression of angiogenic differentiation markers",
    "Protein (translational) confirmation of differentiation markers",
    "Cardiac commitment",
    "Angiogenic commitment"
  ],
  "main_findings": "hAMSCs exposed to an ELF-EMF stimulus (7 Hz, 2.5 µT) showed decreased cell proliferation with no change in metabolic activity, vitality, or apoptotic rate. Exposed cells showed increased mRNA expression of cardiac and angiogenic differentiation markers, confirmed at the translational level, and the authors report ELF-EMF exposure could trigger cardiac commitment and induce both cardiac and angiogenic commitments contemporaneously.",
  "effect_direction": "mixed",
  "limitations": [
    "In vitro study; findings may not translate to in vivo or clinical outcomes",
    "Exposure duration not reported in the abstract",
    "Sample size not reported in the abstract",
    "Details of exposure setup and dosimetry beyond frequency and magnetic flux density not reported in the abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELF-EMF",
    "extremely low frequency",
    "7 Hz",
    "2.5 µT",
    "non-ionizing radiation",
    "human amniotic mesenchymal stromal cells",
    "hAMSCs",
    "cardiac commitment",
    "angiogenic markers",
    "regenerative medicine",
    "cell therapy"
  ],
  "suggested_hubs": [
    {
      "slug": "regenerative-medicine",
      "weight": 0.78,
      "reason": "In vitro ELF-EMF exposure used as a strategy to induce cardiac/angiogenic commitment in stromal cells for cell-therapy protocols."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3390/ijms19082324
PubMed: 30096780

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