Luciferase activity and synthesis of Hsp70 and Hsp90 are insensitive to 50Hz electromagnetic fields.

Life sciences · 1998 · No effect · Low evidence

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No effectLow evidencePaper 5385

Heat shock decreased luciferase activity and induced Hsp70/Hsp90 synthesis, whereas short exposure to 50 Hz electromagnetic fields did not. Cells exposed to EMFs at 300–3000 microT showed luciferase activity similar to control cells, and Hsp70/Hsp90 synthesis…

Study snapshot

PublicationLife sciences · 1998

Study typein_vitro

Reported directionNo effect

Evidence ratingLow

Abstract

The activity of luciferase expressed in transfected 34i cells has been monitored under 50Hz EMF and heat shock. While heat shock decreased the luciferase activity, short exposure to EMFs did not, the luciferase expressed in cells exposed to EMFs at 300-3000 microT showing the same activity as that of control cells. To further analyse whether EMF and thermal stress display similar effects, the relative rate of Hsp90 and Hsp70 synthesis was investigated. Hsp90 and Hsp70 synthesis, while induced by a short thermal stress, was not increased by EMF exposure. These results, contrary to previously proposed similarities between thermal stress and EMF effects at a cellular level, indicate that protein denaturation and misfolding caused by thermal stress and responsible both for a loss of luciferase activity and for an induction of Hsp, are not necessarily induced by exposure to EMFs.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionno_effect

PopulationTransfected 34i cells

Sample sizeNot supplied

ExposureELF · 0.05 MHz · short exposure

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Outcomes measured

Luciferase activity
Hsp70 synthesis
Hsp90 synthesis

Limitations

Sample size not reported in abstract
Exposure duration not specified beyond 'short exposure'
In vitro cell model; generalizability to humans not addressed in abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "short exposure"
  },
  "population": "Transfected 34i cells",
  "sample_size": null,
  "outcomes": [
    "Luciferase activity",
    "Hsp70 synthesis",
    "Hsp90 synthesis"
  ],
  "main_findings": "Heat shock decreased luciferase activity and induced Hsp70/Hsp90 synthesis, whereas short exposure to 50 Hz electromagnetic fields did not. Cells exposed to EMFs at 300–3000 microT showed luciferase activity similar to control cells, and Hsp70/Hsp90 synthesis was not increased by EMF exposure.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in abstract",
    "Exposure duration not specified beyond 'short exposure'",
    "In vitro cell model; generalizability to humans not addressed in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "50 Hz",
    "ELF-EMF",
    "microtesla",
    "luciferase",
    "heat shock",
    "Hsp70",
    "Hsp90",
    "protein denaturation",
    "cell culture",
    "transfected cells"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/s0024-3205(98)00298-7
PubMed: 9718073

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