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Electromagnetic field (EMF) effects on channel activity of nanopore OmpF protein.

European biophysics journal : EBJ · 2009 · Mixed · Low evidence

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MixedLow evidencePaper 4949

At 925 MHz, EMF exposure increased the frequency of channel gating and increased voltage sensitivity in single OmpF porin channels, with effects lasting several milliseconds after the field source was terminated. Channel conductance levels did not change sign…

Study snapshot

PublicationEuropean biophysics journal : EBJ · 2009
Study typein_vitro
Reported directionMixed
Evidence ratingLow

Abstract

In this study, the effects of nonionizing electromagnetic fields (EMF; 925 MHz) on the OmpF porin channel have been characterized at the single-channel level. Channel activity was recorded in real time by the voltage clamp method. Our results showed an increase in the frequency of channel gating and voltage sensitivity. The effects of EMF lasted for several milliseconds after the field source was terminated. However, the conductance levels of channels did not change significantly. Thermal effects of EMF on single-channel properties are a possible cause, based on theoretical evaluation of results that were comparable to those seen in conventional experiments at different temperatures. We conclude that EMF affects both the dynamics and conformation of the channel, either directly by affecting critical amino acid side-chain arrangement, or indirectly, via the electrolyte or the lipid membrane.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro
Effect directionmixed
PopulationNot supplied
Sample sizeNot supplied
ExposureRF · 925 MHz
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

At 925 MHz, EMF exposure increased the frequency of channel gating and increased voltage sensitivity in single OmpF porin channels, with effects lasting several milliseconds after the field source was terminated. Channel conductance levels did not change significantly.

Outcomes measured

  • OmpF porin single-channel gating frequency
  • Voltage sensitivity of channel gating
  • Channel conductance levels
  • Persistence of effects after EMF termination (milliseconds)

Limitations

  • No sample size reported in abstract
  • Exposure metrics beyond frequency (e.g., SAR, field strength) not reported in abstract
  • Mechanism not established; thermal effects proposed based on theoretical evaluation
View raw extracted JSON
{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": 925,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "OmpF porin single-channel gating frequency",
    "Voltage sensitivity of channel gating",
    "Channel conductance levels",
    "Persistence of effects after EMF termination (milliseconds)"
  ],
  "main_findings": "At 925 MHz, EMF exposure increased the frequency of channel gating and increased voltage sensitivity in single OmpF porin channels, with effects lasting several milliseconds after the field source was terminated. Channel conductance levels did not change significantly.",
  "effect_direction": "mixed",
  "limitations": [
    "No sample size reported in abstract",
    "Exposure metrics beyond frequency (e.g., SAR, field strength) not reported in abstract",
    "Mechanism not established; thermal effects proposed based on theoretical evaluation"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "nonionizing electromagnetic fields",
    "RF",
    "925 MHz",
    "OmpF",
    "porin",
    "nanopore",
    "single-channel recording",
    "voltage clamp",
    "gating",
    "voltage sensitivity",
    "conductance",
    "thermal effects"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1007/s00249-009-0511-4
PubMed: 19603160

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