Proliferation and differentiation of rat bone marrow stem cells by 400μT electromagnetic field.

Neuroscience letters · 2016 · Mixed · Low evidence

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MixedLow evidencePaper 4725

Study snapshot

PublicationNeuroscience letters · 2016

Study typeanimal

Reported directionMixed

Evidence ratingLow

Abstract

The interaction between environment electromagnetic field (EMF) and cells can effect on various physiological processes. EMF as an external inducing factor, could effect on proliferation or differentiation of cells. The purpose of this study was to evaluate the influence of the electromagnetic field on the viability, proliferation and differentiation rate of bone marrow stem cells (BMSCs) to neuron. BMSCs were obtained from 42 adult male rats. The cells incubated and cultured in 96-wells and 6-wells plates and exposed to electromagnetic field (40 or 400μT) with a selected waveform: AC (alternative current), rectified half wave (RHW) and rectified full wave (RFW), for a week. To assess the viability and proliferation rate of treated cells, MTT assay was done, and then immunocytochemistry staining Neu N was used to evaluate cell differentiation to neuron. Results showed that EMF decreases the viability and proliferation in treated groups. But in AC group's reduction was significant. Minimum viability and proliferation rate was observed in RHW 400μT group compared with sham. Immunocytochemistry showed that EMF can induce BMSC differentiation into neuron in AC 400μT and RFW 400μT. Evidences of this research support the hypothesis that EMF can induce differentiation of BMSCs to neuron.

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Study typeanimal

Effect directionmixed

PopulationBone marrow stem cells (BMSCs) obtained from 42 adult male rats

Sample size42

ExposureELF · for a week

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Rat bone marrow stem cells exposed for one week to electromagnetic fields of 40 or 400 μT (AC, rectified half wave, or rectified full wave) showed decreased viability and proliferation versus sham; the reduction was reported as significant in the AC group, with the minimum viability/proliferation in the RHW 400 μT group. Immunocytochemistry (NeuN) indicated EMF-induced differentiation into neuron in AC 400 μT and RFW 400 μT conditions.

Outcomes measured

Cell viability (MTT assay)
Cell proliferation (MTT assay)
Differentiation of BMSCs to neuron (NeuN immunocytochemistry)

View raw extracted JSON

{
  "study_type": "animal",
  "exposure": {
    "band": "ELF",
    "source": "other",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "for a week"
  },
  "population": "Bone marrow stem cells (BMSCs) obtained from 42 adult male rats",
  "sample_size": 42,
  "outcomes": [
    "Cell viability (MTT assay)",
    "Cell proliferation (MTT assay)",
    "Differentiation of BMSCs to neuron (NeuN immunocytochemistry)"
  ],
  "main_findings": "Rat bone marrow stem cells exposed for one week to electromagnetic fields of 40 or 400 μT (AC, rectified half wave, or rectified full wave) showed decreased viability and proliferation versus sham; the reduction was reported as significant in the AC group, with the minimum viability/proliferation in the RHW 400 μT group. Immunocytochemistry (NeuN) indicated EMF-induced differentiation into neuron in AC 400 μT and RFW 400 μT conditions.",
  "effect_direction": "mixed",
  "limitations": [],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electromagnetic field",
    "ELF-EMF",
    "400 μT",
    "40 μT",
    "AC waveform",
    "rectified half wave",
    "rectified full wave",
    "bone marrow stem cells",
    "viability",
    "proliferation",
    "neuronal differentiation",
    "NeuN",
    "rat"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/j.neulet.2015.11.044
PubMed: 26639423

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