Improved resolution in the gel electrophoresis of proteins by a periodically interrupted electric field.

Journal of biochemical and biophysical methods · 1996 · Benefit · Low evidence

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BenefitLow evidencePaper 4172

Study snapshot

PublicationJournal of biochemical and biophysical methods · 1996

Study typeengineering

Reported directionBenefit

Evidence ratingLow

Abstract

The capability of the commercial gel electrophoresis apparatus with intermittent scanning of fluorescence (HPGE-1000, LabIntelligence) to provide time-dependent zone dispersion allows one to quantitate resolution. Using a model protein separation, that between phycoerythrin and fluorescein-labeled conalbumin, resolution was compared between separations conducted at a constant field strength of 80 V/cm and one conducted in 10-s pulses of the same field strength, interrupted periodically by 120 s in the absence of an electric field. Resolution was improved by a factor of two in the discontinuous application of the electric field compared to that obtained in its continuous application. Similarly, the intermittent application of 80 V/cm for 10 s, followed by 120-s pauses, gave rise to twice the resolution obtained from a continuous application of 7 V/cm.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeengineering

Effect directionbenefit

PopulationNot supplied

Sample sizeNot supplied

Exposure10-s pulses interrupted by 120 s without field (also compared to continuous application)

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In a model protein separation (phycoerythrin vs fluorescein-labeled conalbumin), applying an electric field discontinuously (80 V/cm in 10-s pulses with 120-s pauses) produced about twice the resolution compared with continuous application of 80 V/cm. The same intermittent 80 V/cm protocol also yielded about twice the resolution compared with continuous application of 7 V/cm.

Outcomes measured

Protein separation resolution (zone dispersion-based quantitated resolution)

Limitations

No sample size or number of runs reported in the abstract
Study is a methodological/engineering application in gel electrophoresis rather than a health-effects study
Limited to a specific model protein separation and specific apparatus/protocol described

View raw extracted JSON

{
  "study_type": "engineering",
  "exposure": {
    "band": null,
    "source": "gel electrophoresis apparatus (electric field)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "10-s pulses interrupted by 120 s without field (also compared to continuous application)"
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "Protein separation resolution (zone dispersion-based quantitated resolution)"
  ],
  "main_findings": "In a model protein separation (phycoerythrin vs fluorescein-labeled conalbumin), applying an electric field discontinuously (80 V/cm in 10-s pulses with 120-s pauses) produced about twice the resolution compared with continuous application of 80 V/cm. The same intermittent 80 V/cm protocol also yielded about twice the resolution compared with continuous application of 7 V/cm.",
  "effect_direction": "benefit",
  "limitations": [
    "No sample size or number of runs reported in the abstract",
    "Study is a methodological/engineering application in gel electrophoresis rather than a health-effects study",
    "Limited to a specific model protein separation and specific apparatus/protocol described"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "gel electrophoresis",
    "electric field",
    "pulsed field",
    "intermittent field",
    "protein separation",
    "resolution",
    "zone dispersion",
    "fluorescence scanning"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1016/s0165-022x(96)00021-8
PubMed: 9029260

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