Electric field directed nucleic acid hybridization on microchips.

Nucleic acids research · 1997 · Unclear · Insufficient evidence

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UnclearInsufficient evidencePaper 4128

Study snapshot

PublicationNucleic acids research · 1997

Study typeengineering

Reported directionUnclear

Evidence ratingInsufficient

Abstract

Selection and adjustment of proper physical parameters enables rapid DNA transport, site selective concentration, and accelerated hybridization reactions to be carried out on active microelectronic arrays. These physical parameters include DC current, voltage, solution conductivity and buffer species. Generally, at any given current and voltage level, the transport or mobility of DNA is inversely proportional to electrolyte or buffer conductivity. However, only a subset of buffer species produce both rapid transport, site specific concentration and accelerated hybridization. These buffers include zwitterionic and low conductivity species such as: d- and l-histidine; 1- and 3-methylhistidines; carnosine; imidazole; pyridine; and collidine. In contrast, buffers such as glycine, beta-alanine and gamma-amino-butyric acid (GABA) produce rapid transport and site selective concentration but do not facilitate hybridization. Our results suggest that the ability of these buffers (histidine, etc.) to facilitate hybridization appears linked to their ability to provide electric field concentration of DNA; to buffer acidic conditions present at the anode; and in this process acquire a net positive charge which then shields or diminishes repulsion between the DNA strands, thus promoting hybridization.

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Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeengineering

Effect directionunclear

PopulationNot supplied

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthInsufficientConfidence: 74% · Peer reviewed: yes

Main findings

Adjusting physical parameters (DC current, voltage, solution conductivity, buffer species) enabled rapid DNA transport, site-selective concentration, and accelerated hybridization on active microelectronic arrays. DNA mobility was generally inversely proportional to buffer conductivity at a given current/voltage. Only certain low-conductivity/zwitterionic buffers (e.g., histidine-related buffers, imidazole, pyridine, collidine) supported both transport/concentration and accelerated hybridization, whereas others (e.g., glycine, beta-alanine, GABA) supported transport/concentration but not hybridization.

Outcomes measured

DNA transport/mobility
site-selective DNA concentration
accelerated nucleic acid hybridization on microelectronic arrays
buffer species effects on hybridization/transport

View raw extracted JSON

{
  "study_type": "engineering",
  "exposure": {
    "band": null,
    "source": "microelectronic arrays (active microchips)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": null,
  "sample_size": null,
  "outcomes": [
    "DNA transport/mobility",
    "site-selective DNA concentration",
    "accelerated nucleic acid hybridization on microelectronic arrays",
    "buffer species effects on hybridization/transport"
  ],
  "main_findings": "Adjusting physical parameters (DC current, voltage, solution conductivity, buffer species) enabled rapid DNA transport, site-selective concentration, and accelerated hybridization on active microelectronic arrays. DNA mobility was generally inversely proportional to buffer conductivity at a given current/voltage. Only certain low-conductivity/zwitterionic buffers (e.g., histidine-related buffers, imidazole, pyridine, collidine) supported both transport/concentration and accelerated hybridization, whereas others (e.g., glycine, beta-alanine, GABA) supported transport/concentration but not hybridization.",
  "effect_direction": "unclear",
  "limitations": [],
  "evidence_strength": "insufficient",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electric field",
    "DC current",
    "voltage",
    "microelectronic arrays",
    "microchips",
    "DNA transport",
    "hybridization",
    "buffer conductivity",
    "histidine",
    "imidazole",
    "pyridine",
    "collidine",
    "zwitterionic buffers"
  ],
  "suggested_hubs": [
    {
      "slug": "engineering",
      "weight": 0.6,
      "reason": "Study describes electric-field-directed DNA transport and hybridization on active microelectronic arrays (microchips)."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1093/nar/25.24.4907
PubMed: 9396795

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