[Effects of magnetic field on MAPK signaling pathways of human retinal pigment epithelial cells bound with beads in vitro].

[Zhonghua yan ke za zhi] Chinese journal of ophthalmology · 2006 · Mixed · Low evidence

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MixedLow evidencePaper 3492

Study snapshot

Publication[Zhonghua yan ke za zhi] Chinese journal of ophthalmology · 2006

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

OBJECTIVE: To observe the changes of mitogen activated protein kinase (MAPK) signaling pathways of human retinal pigment epithelial cells( RPEs) bound with beads under stretch caused by the force from magnetic field in vitro. METHODS: Ferric oxide microparticles, coAted with collagen, were added to dishes containing substrate-attached RPEs. After incubation, the cell layer bound with beads was laid in a magnetic field, the cells were stretched by the magnetic force. The activation status of the extracellular signal-regulated protein kinase (ERK), c-jun N-terminal kinase (JNK), and p38 in RPEs was determined over a time course from 3 to 30 minutes with western-blot analysis. To examine the role of p38 kinase in the response to stretching, cells were grown for 30 minutes in the presence or absence of inhibitor of p38 (SB203580). The changes of the expression of active p38 kinase were observed with fluorescence staining. RESULTS: Total ERK, JNK, and p38 were detected in RPEs. Active ERK and p38 were detected but active JNK was not detected. Activation of ERK was unchanged during the time course. In contrast, p38 activation was barely detected in the normal cells, but this stress-activated protein kinase exhibited a robust activation after 5 minutes in the magnetic field. SB203580 blocked the p38 activation during stretch stimulation. The stretch stimulation also increased the fluorescence staining of active p38. CONCLUSION: A magnetic field can affect RPEs bound with beads. The effect may be partially through p38 signaling pathway.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman retinal pigment epithelial cells (RPEs) in vitro

Sample sizeNot supplied

Exposure3–30 minutes (time course); inhibitor condition 30 minutes

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Total ERK, JNK, and p38 were detected in RPEs; active ERK and p38 were detected but active JNK was not detected. ERK activation was unchanged over 3–30 minutes, while p38 activation was barely detected in normal cells and showed robust activation after 5 minutes in the magnetic field; SB203580 blocked p38 activation and stretch stimulation increased fluorescence staining of active p38.

Outcomes measured

ERK activation status
JNK activation status
p38 activation status
Effect of p38 inhibitor SB203580 on p38 activation
Fluorescence staining of active p38

Limitations

Magnetic field parameters (e.g., strength, frequency) not reported in abstract
In vitro model using bead-bound cells and magnetic-force stretching may not generalize to typical environmental/occupational magnetic-field exposures
Sample size and replication not reported in abstract
Short exposure window (minutes) only

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": null,
    "source": "magnetic field (in vitro stretching via magnetic force on ferric oxide microparticles bound to cells)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "3–30 minutes (time course); inhibitor condition 30 minutes"
  },
  "population": "Human retinal pigment epithelial cells (RPEs) in vitro",
  "sample_size": null,
  "outcomes": [
    "ERK activation status",
    "JNK activation status",
    "p38 activation status",
    "Effect of p38 inhibitor SB203580 on p38 activation",
    "Fluorescence staining of active p38"
  ],
  "main_findings": "Total ERK, JNK, and p38 were detected in RPEs; active ERK and p38 were detected but active JNK was not detected. ERK activation was unchanged over 3–30 minutes, while p38 activation was barely detected in normal cells and showed robust activation after 5 minutes in the magnetic field; SB203580 blocked p38 activation and stretch stimulation increased fluorescence staining of active p38.",
  "effect_direction": "mixed",
  "limitations": [
    "Magnetic field parameters (e.g., strength, frequency) not reported in abstract",
    "In vitro model using bead-bound cells and magnetic-force stretching may not generalize to typical environmental/occupational magnetic-field exposures",
    "Sample size and replication not reported in abstract",
    "Short exposure window (minutes) only"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "magnetic field",
    "retinal pigment epithelial cells",
    "RPE",
    "MAPK",
    "ERK",
    "JNK",
    "p38",
    "mechanical stretch",
    "ferric oxide microparticles",
    "SB203580",
    "western blot",
    "fluorescence staining"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: Not supplied
PubMed: 17415968

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