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Influences of electric field on living cells in a charged water-in-oil droplet under electrophoretic actuation.

Biomicrofluidics · 2011 · No effect · Low evidence

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No effectLow evidencePaper 3196

In experiments with charged aqueous droplets undergoing electrophoretic motion under strong electric fields (1–3 kV/cm), the authors report no noticeable influence of the electric field or silicone oil on cell viability or proliferation across the tested fiel…

Study snapshot

PublicationBiomicrofluidics · 2011
Study typeother
Reported directionNo effect
Evidence ratingLow

Abstract

We experimentally investigate the effects of high electric field on living cells inside a charged droplet under electrophoretic actuation. When an aqueous droplet suspended in a dielectric liquid contacts with electrified electrode, the droplet acquires charge. This charged droplet undergoes electrophoretic motion under strong electric field (1-3 kV/cm), which can be used as a droplet manipulation method in biomicrofluidic applications. However, because strong electric field and use of dielectric oil can be a harmful environment for living cells, the biological feasibilities have been tested. Trypan blue test and cell growth test have been performed to check the viability and proliferation of cells in a droplet under various electric field strengths and actuation times. We have not observed any noticeable influence of electric field and silicone oil on the viability and proliferation of cells, which indicates that electrophoresis could be safely used as a manipulation method for a droplet containing living biological system.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeother
Effect directionno_effect
Populationliving cells in an aqueous droplet suspended in dielectric liquid (silicone oil)
Sample sizeNot supplied
Exposurevarious electric field strengths and actuation times
Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

In experiments with charged aqueous droplets undergoing electrophoretic motion under strong electric fields (1–3 kV/cm), the authors report no noticeable influence of the electric field or silicone oil on cell viability or proliferation across the tested field strengths and actuation times.

Outcomes measured

  • cell viability (Trypan blue test)
  • cell proliferation/growth (cell growth test)

Limitations

  • Sample size not reported in abstract
  • Cell type(s) not specified in abstract
  • Details of exposure duration/actuation times and experimental conditions not provided in abstract
  • Only viability and growth/proliferation endpoints mentioned in abstract
View raw extracted JSON
{
  "study_type": "other",
  "exposure": {
    "band": null,
    "source": "laboratory electric field (electrophoretic actuation in charged water-in-oil droplet)",
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "various electric field strengths and actuation times"
  },
  "population": "living cells in an aqueous droplet suspended in dielectric liquid (silicone oil)",
  "sample_size": null,
  "outcomes": [
    "cell viability (Trypan blue test)",
    "cell proliferation/growth (cell growth test)"
  ],
  "main_findings": "In experiments with charged aqueous droplets undergoing electrophoretic motion under strong electric fields (1–3 kV/cm), the authors report no noticeable influence of the electric field or silicone oil on cell viability or proliferation across the tested field strengths and actuation times.",
  "effect_direction": "no_effect",
  "limitations": [
    "Sample size not reported in abstract",
    "Cell type(s) not specified in abstract",
    "Details of exposure duration/actuation times and experimental conditions not provided in abstract",
    "Only viability and growth/proliferation endpoints mentioned in abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "electric field",
    "electrophoresis",
    "electrophoretic actuation",
    "charged droplet",
    "water-in-oil droplet",
    "silicone oil",
    "biomicrofluidics",
    "cell viability",
    "Trypan blue",
    "cell growth",
    "proliferation"
  ],
  "suggested_hubs": []
}
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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1063/1.3665222
PubMed: 22662063

Read the original paper →

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