Sweeping of hydrophobic amines under inhomogeneous electric field and low surfactant concentration in micellar electrokinetic chromatography.

Electrophoresis · 2016 · Unclear · Insufficient evidence

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UnclearInsufficient evidencePaper 2931

Study snapshot

PublicationElectrophoresis · 2016

Study typeother

Reported directionUnclear

Evidence ratingInsufficient

Abstract

The influence of sample matrix on sample sweeping in MEKC was examined in the presented manuscript. Significant focusing effect was observed for relatively hydrophobic cationic compounds (emetine, strychnine and quinine) using high ionic strength sample matrix (900 mM H3 PO4 /720 mM Tris) which conductivity was about ninefold higher than utilized BGE. Moreover, the results were obtained using BGE composed of comparatively low surfactant concentration (10 mM SDS) and 40 mM H3 PO4 /32 mM Tris buffer solution. About 200 to 300-fold preconcentration of analytes was reached with the presented method. Basing on experimental results and computer simulation using Simul5 software, hypothetical mechanism of observed phenomenon was proposed.

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typeother

Effect directionunclear

PopulationNot supplied

Sample sizeNot supplied

ExposureNot supplied

Evidence strengthInsufficientConfidence: 74% · Peer reviewed: yes

Main findings

In MEKC, a significant focusing (sweeping) effect for relatively hydrophobic cationic compounds (emetine, strychnine, quinine) was observed using a high ionic strength sample matrix with conductivity about ninefold higher than the background electrolyte (BGE). Using a BGE with low surfactant concentration (10 mM SDS), the method achieved approximately 200–300-fold analyte preconcentration; a hypothetical mechanism was proposed based on experiments and Simul5 simulations.

Outcomes measured

Sample sweeping/focusing in micellar electrokinetic chromatography (MEKC)
Analyte preconcentration factor

View raw extracted JSON

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  "study_type": "other",
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  },
  "population": null,
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  "outcomes": [
    "Sample sweeping/focusing in micellar electrokinetic chromatography (MEKC)",
    "Analyte preconcentration factor"
  ],
  "main_findings": "In MEKC, a significant focusing (sweeping) effect for relatively hydrophobic cationic compounds (emetine, strychnine, quinine) was observed using a high ionic strength sample matrix with conductivity about ninefold higher than the background electrolyte (BGE). Using a BGE with low surfactant concentration (10 mM SDS), the method achieved approximately 200–300-fold analyte preconcentration; a hypothetical mechanism was proposed based on experiments and Simul5 simulations.",
  "effect_direction": "unclear",
  "limitations": [],
  "evidence_strength": "insufficient",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "micellar electrokinetic chromatography",
    "MEKC",
    "sample sweeping",
    "focusing effect",
    "preconcentration",
    "hydrophobic amines",
    "inhomogeneous electric field",
    "SDS",
    "ionic strength",
    "Simul5"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/elps.201500436
PubMed: 27135308

Read the original paper →