[Genic and non-genic regulation of low frequence pulsed electromagnetic fields on osteoblasts differentiation].

Zhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2016 · Benefit · Low evidence

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Study snapshot

PublicationZhejiang da xue xue bao. Yi xue ban = Journal of Zhejiang University. Medical sciences · 2016

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

To study the genic and non-genic regulation of 50 Hz 0.6 mT pulsed electromagnenic fields (PEMF) on rat calvarial osteoblasts (ROB) differentiation. ROBs were achieved by enzyme digestion, and treated with 50 Hz 0.6 mT PEMFs for 1.5 hours after subculture. The alkaline phosphatase (ALP) activity, mRNA transcription of ALP, Runx2 and OSX and protein expression of Runx2 and OSX were detected at 0, 3, 6, 9 and 12 hours after PEMF treatment. The ALP activity at 3 hours after treatment was significantly higher than that in the control(<0.01), while the mRNA transcription of ALP began to increase at 6 hours after treatment. The mRNA transcription of Runx2 increased immediately after treatment and regressed at 6 hours, then increased again. The protein expression of it corresponded but with a little lag. The mRNA transcription of OSX also raised instantly after treatment, then returned to the level of control at 6 hours, and lower than control at 12 hours significantly. The protein expression of it also corresponded but with a bit delay. There are genic regulation for the protein expression of Runx2 and OSX, and non-genic regulation for the ALP activity on the process of 50 Hz 0.6 mT PEMFs prompts ROBs differentiation.

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationRat calvarial osteoblasts (ROB) in culture

Sample sizeNot supplied

ExposureELF · 0.05 MHz · 1.5 hours (single treatment)

Evidence strengthLowConfidence: 78% · Peer reviewed: yes

Main findings

Rat calvarial osteoblasts exposed to 50 Hz 0.6 mT PEMF for 1.5 hours showed increased ALP activity at 3 hours post-treatment versus control, with ALP mRNA increasing starting at 6 hours. Runx2 and OSX mRNA and protein expression changed over time after exposure (early increases with later regression/changes), and the authors conclude PEMF promotes osteoblast differentiation with both genic (Runx2/OSX protein expression) and non-genic (ALP activity) regulation.

Outcomes measured

Alkaline phosphatase (ALP) activity
ALP mRNA transcription
Runx2 mRNA transcription
OSX mRNA transcription
Runx2 protein expression
OSX protein expression

Limitations

Sample size not reported in abstract
In vitro rat cell model; generalizability to humans not addressed
Short follow-up window (0–12 hours)
Limited outcomes focused on early differentiation markers; no functional bone formation endpoints reported

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": "pulsed electromagnetic fields (PEMF) exposure system",
    "frequency_mhz": 0.05,
    "sar_wkg": null,
    "duration": "1.5 hours (single treatment)"
  },
  "population": "Rat calvarial osteoblasts (ROB) in culture",
  "sample_size": null,
  "outcomes": [
    "Alkaline phosphatase (ALP) activity",
    "ALP mRNA transcription",
    "Runx2 mRNA transcription",
    "OSX mRNA transcription",
    "Runx2 protein expression",
    "OSX protein expression"
  ],
  "main_findings": "Rat calvarial osteoblasts exposed to 50 Hz 0.6 mT PEMF for 1.5 hours showed increased ALP activity at 3 hours post-treatment versus control, with ALP mRNA increasing starting at 6 hours. Runx2 and OSX mRNA and protein expression changed over time after exposure (early increases with later regression/changes), and the authors conclude PEMF promotes osteoblast differentiation with both genic (Runx2/OSX protein expression) and non-genic (ALP activity) regulation.",
  "effect_direction": "benefit",
  "limitations": [
    "Sample size not reported in abstract",
    "In vitro rat cell model; generalizability to humans not addressed",
    "Short follow-up window (0–12 hours)",
    "Limited outcomes focused on early differentiation markers; no functional bone formation endpoints reported"
  ],
  "evidence_strength": "low",
  "confidence": 0.78,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "pulsed electromagnetic fields",
    "PEMF",
    "50 Hz",
    "0.6 mT",
    "extremely low frequency",
    "osteoblast",
    "rat calvarial osteoblasts",
    "ALP",
    "Runx2",
    "OSX",
    "differentiation"
  ],
  "suggested_hubs": [
    {
      "slug": "elf-emf",
      "weight": 0.9,
      "reason": "Exposure is 50 Hz (ELF) PEMF at 0.6 mT."
    }
  ]
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.3785/j.issn.1008-9292.2016.11.02
PubMed: 28247598

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