ELF EMF induces neural differentiation of hBM-MSCs through regulation of (Zn)-metallothionein-3

2017 · Benefit · Low evidence

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BenefitLow evidencePaper 2305

Study snapshot

Publicationmanual · 2017

Study typein_vitro

Reported directionBenefit

Evidence ratingLow

Abstract

ELF EMF induces neural differentiation of hBM-MSCs through regulation of (Zn)-metallothionein-3 Aikins AR, Hong SW, Kim HJ, Yoon CH, Chung JH, Kim M, Kim CW. Extremely low-frequency electromagnetic field induces neural differentiation of hBM-MSCs through regulation of (Zn)-metallothionein-3. Bioelectromagnetics. 2017 Mar 29. doi: 10.1002/bem.22046. Abstract Extremely low-frequency electromagnetic field (ELFEMF) can stimulate neural differentiation in human bone marrow-derived mesenchymal cells (hBM-MSCs), and this provides an opportunity for research on neurodegenerative diseases such as Alzheimer's disease (AD). Metallothionein-3 (MT3), an isoform of the metal- binding proteins, metallothioneins, involved in maintaining intracellular zinc (Zn) homeostasis and the deregulation of zinc homeostasis, has separately been implicated in AD. Here, we investigated the effect of ELFEMF-induced neural differentiation of hBM-MSCs on Zn-MT3 homeostatic interaction. Exposure to ELFEMF induced neural differentiation of hBM-MSCs, which was characterized by decreased proliferation and enhanced neural-like morphology. We observed expression of neuronal markers such as β-tubulin3, pleiotrophin, and neurofilament-M at the mRNA level and MAP2 at the protein level. ELFEMF-induced neural differentiation correlated with decreased expression of metal-response element-transcription factor 1 and MT3, as well as decreased intracellular Zn concentration. In addition, upregulation of dihydropyrimidinase-related protein 2 was observed, but there was no change in γ-enolase expression. These data indicate a possible regulatory mechanism for MT3 during neural differentiation. Our findings provide considerable insight into molecular mechanisms involved in neural differentiation, which is useful for developing new treatments for neurodegenerative diseases. ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionbenefit

PopulationHuman bone marrow-derived mesenchymal stem cells (hBM-MSCs)

Sample sizeNot supplied

ExposureELF

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

Exposure to extremely low-frequency electromagnetic field (ELFEMF) induced neural differentiation of hBM-MSCs, characterized by decreased proliferation and enhanced neural-like morphology, with increased neuronal marker expression (mRNA: β-tubulin3, pleiotrophin, neurofilament-M; protein: MAP2). ELFEMF-induced differentiation correlated with decreased MTF1 and MT3 expression and decreased intracellular Zn concentration; dihydropyrimidinase-related protein 2 was upregulated and γ-enolase was unchanged.

Outcomes measured

Neural differentiation of hBM-MSCs
Cell proliferation
Neural-like morphology
mRNA expression of neuronal markers (β-tubulin3, pleiotrophin, neurofilament-M)
Protein expression of MAP2
Expression of metal-response element-transcription factor 1 (MTF1)
Expression of metallothionein-3 (MT3)
Intracellular zinc (Zn) concentration
Expression of dihydropyrimidinase-related protein 2
Expression of γ-enolase

Limitations

Exposure parameters (e.g., frequency, field strength, duration) not reported in the provided abstract
In vitro study; clinical relevance not established in the provided abstract
Sample size and replication details not reported in the provided abstract

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human bone marrow-derived mesenchymal stem cells (hBM-MSCs)",
  "sample_size": null,
  "outcomes": [
    "Neural differentiation of hBM-MSCs",
    "Cell proliferation",
    "Neural-like morphology",
    "mRNA expression of neuronal markers (β-tubulin3, pleiotrophin, neurofilament-M)",
    "Protein expression of MAP2",
    "Expression of metal-response element-transcription factor 1 (MTF1)",
    "Expression of metallothionein-3 (MT3)",
    "Intracellular zinc (Zn) concentration",
    "Expression of dihydropyrimidinase-related protein 2",
    "Expression of γ-enolase"
  ],
  "main_findings": "Exposure to extremely low-frequency electromagnetic field (ELFEMF) induced neural differentiation of hBM-MSCs, characterized by decreased proliferation and enhanced neural-like morphology, with increased neuronal marker expression (mRNA: β-tubulin3, pleiotrophin, neurofilament-M; protein: MAP2). ELFEMF-induced differentiation correlated with decreased MTF1 and MT3 expression and decreased intracellular Zn concentration; dihydropyrimidinase-related protein 2 was upregulated and γ-enolase was unchanged.",
  "effect_direction": "benefit",
  "limitations": [
    "Exposure parameters (e.g., frequency, field strength, duration) not reported in the provided abstract",
    "In vitro study; clinical relevance not established in the provided abstract",
    "Sample size and replication details not reported in the provided abstract"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "ELFEMF",
    "extremely low-frequency electromagnetic field",
    "hBM-MSCs",
    "human bone marrow-derived mesenchymal stem cells",
    "neural differentiation",
    "metallothionein-3",
    "MT3",
    "zinc homeostasis",
    "MTF1",
    "neuronal markers",
    "MAP2",
    "Bioelectromagnetics"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1002/bem.22046
PubMed: Not supplied

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