ELF EMF promotes astrocytic differentiation of human bone marrow stem cells by modulating SIRT1 expression

2017 · Mixed · Low evidence

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MixedLow evidencePaper 2302

Study snapshot

Publicationmanual · 2017

Study typein_vitro

Reported directionMixed

Evidence ratingLow

Abstract

ELF EMF promotes astrocytic differentiation of human bone marrow stem cells by modulating SIRT1 expression Jeong WY, Kim JB, Kim HJ, Kim CW. Extremely low-frequency electromagnetic field promotes astrocytic differentiation of human bone marrow mesenchymal stem cells by modulating SIRT1 expression. Biosci Biotechnol Biochem. 2017 Mar 29:1-7. doi: 10.1080/09168451.2017.1308243. Abstract It has been shown that extremely low-frequency electromagnetic fields (ELFMF) affect regulation of cell fate and differentiation. Thus, the aim of this study was to investigate the role of ELFMFs in the enhancement of astrocytic differentiation. ELFMF exposure reduced the rate of proliferation and enhanced astrocytic differentiation. The ELFMF-treated cells showed increased levels of the astrocyte marker (GFAP), while those of the early neuronal marker (Nestin) and stemness marker (OCT3/4) were downregulated. The reactive oxygen species (ROS) level was observed to be significantly elevated after ELFMF exposure, which strengthens the modulatory role of SIRT1 and SIRT1 downstream molecules (TLE1, HES1, and MASH1) during astrocytic differentiation. After nicotinamide (5 mM) mediated inhibition of SIRT1, levels of TLE1, HES1, and MASH1 were examined; TLE1 was significantly upregulated and MASH1 was downregulated. These results suggest that ELFMFs induce astrocytic differentiation through activation of SIRT1 and SIRT1 downstream molecules. ncbi.nlm.nih.gov

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AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionmixed

PopulationHuman bone marrow mesenchymal stem cells (in vitro)

Sample sizeNot supplied

ExposureELF

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

ELF EMF exposure reduced proliferation and enhanced astrocytic differentiation of human bone marrow mesenchymal stem cells, with increased GFAP and decreased Nestin and OCT3/4. ROS levels were significantly elevated after exposure, and results were described as consistent with ELFMF-induced astrocytic differentiation via activation/modulation of SIRT1 and downstream molecules (TLE1, HES1, MASH1); with nicotinamide inhibition of SIRT1, TLE1 increased and MASH1 decreased.

Outcomes measured

Cell proliferation rate
Astrocytic differentiation
GFAP (astrocyte marker) expression
Nestin (early neuronal marker) expression
OCT3/4 (stemness marker) expression
Reactive oxygen species (ROS) levels
SIRT1 expression/activity and downstream molecules (TLE1, HES1, MASH1)
Effects of SIRT1 inhibition with nicotinamide (5 mM) on TLE1, HES1, MASH1

Limitations

No exposure parameters reported (e.g., frequency, field strength, duration).
In vitro study; findings may not generalize to humans in vivo.
Sample size and experimental replication not reported in the abstract.

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "ELF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": null
  },
  "population": "Human bone marrow mesenchymal stem cells (in vitro)",
  "sample_size": null,
  "outcomes": [
    "Cell proliferation rate",
    "Astrocytic differentiation",
    "GFAP (astrocyte marker) expression",
    "Nestin (early neuronal marker) expression",
    "OCT3/4 (stemness marker) expression",
    "Reactive oxygen species (ROS) levels",
    "SIRT1 expression/activity and downstream molecules (TLE1, HES1, MASH1)",
    "Effects of SIRT1 inhibition with nicotinamide (5 mM) on TLE1, HES1, MASH1"
  ],
  "main_findings": "ELF EMF exposure reduced proliferation and enhanced astrocytic differentiation of human bone marrow mesenchymal stem cells, with increased GFAP and decreased Nestin and OCT3/4. ROS levels were significantly elevated after exposure, and results were described as consistent with ELFMF-induced astrocytic differentiation via activation/modulation of SIRT1 and downstream molecules (TLE1, HES1, MASH1); with nicotinamide inhibition of SIRT1, TLE1 increased and MASH1 decreased.",
  "effect_direction": "mixed",
  "limitations": [
    "No exposure parameters reported (e.g., frequency, field strength, duration).",
    "In vitro study; findings may not generalize to humans in vivo.",
    "Sample size and experimental replication not reported in the abstract."
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "extremely low-frequency electromagnetic field",
    "ELF EMF",
    "ELFMF",
    "astrocytic differentiation",
    "human bone marrow mesenchymal stem cells",
    "GFAP",
    "Nestin",
    "OCT3/4",
    "ROS",
    "SIRT1",
    "TLE1",
    "HES1",
    "MASH1",
    "nicotinamide"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.1080/09168451.2017.1308243
PubMed: Not supplied

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