Assessment of the effects of radiofrequency radiation on human colon epithelium cells

2019 · Harm · Low evidence

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HarmLow evidencePaper 1839

Study snapshot

Publicationmanual · 2019

Study typein_vitro

Reported directionHarm

Evidence ratingLow

Abstract

Assessment of the effects of radiofrequency radiation on human colon epithelium cells Tomruk A, Terzi YK, Guler GO. Assessment of the effects of radiofrequency radiation on human colon epithelium cells. Bratisl Lek Listy. 2019;120(4):299-308. doi: 10.4149/BLL_2019_050. Abstract OBJECTIVES: The aim of the study was to investigate the possible effects of radiofrequency radiation (RFR) at different frequencies for different exposure durations on caspase-dependent apoptosis pathways in human colon adenocarcinoma (HT-29). METHODS: HT-29 cells were exposed to 1800 MHz; 2100 MHz and 2600 MHz RFR for 3 h cont., 6 h int. and 6 h cont.. Cell viability measurements were performed by Trypan Blue exclusion assay and the gene expressions of CASP8, CASP9, CASP3 and CASP12 were analyzed using qRT-PCR. RESULTS: Exposure to 2100 MHz RFR for all 3 durations of exposures was more effective for the ratio of the number of viable HT-29 cells w.r.t 1800 MHz RFR and 2600 MHz RFR exposures. After 2100 MHz RFR exposure, caspase activation increased significantly (for 3h cont. and 6 h int. exposures CASP8 and CASP9 levels; for 6 h cont. exposure CASP3 levels) (p 0.05). CONCLUSION: Decreases in the cell viability of HT-29 cells for certain frequencies and also durations are consistent with significant increases in caspase activations. The results of caspase activation after 1800 MHz or 2600 MHz RFR exposures can be interpreted as the activation of different types of cell death pathway by caspase signaling cascades (Fig. 15, Ref. 56). ncbi.nlm.nih.gov

Machine Enhanced Logic

AI evidence extraction

Structured fields below were generated from the paper’s abstract and metadata to make the evidence easier to inspect. Automated extraction may be incomplete or incorrect; verify important details against the original paper.

Study typein_vitro

Effect directionharm

PopulationHuman colon adenocarcinoma (HT-29) cells

Sample sizeNot supplied

ExposureRF · 3 h continuous; 6 h intermittent; 6 h continuous

Evidence strengthLowConfidence: 74% · Peer reviewed: yes

Main findings

HT-29 cells exposed to 1800, 2100, and 2600 MHz radiofrequency radiation showed frequency- and duration-dependent changes. Exposure at 2100 MHz for all three exposure durations was reported as more effective on the ratio of viable cells compared with 1800 and 2600 MHz, and 2100 MHz exposure was associated with significant increases in caspase activation (CASP8/CASP9 for 3 h continuous and 6 h intermittent; CASP3 for 6 h continuous).

Outcomes measured

Cell viability (Trypan Blue exclusion assay)
Gene expression of CASP8, CASP9, CASP3, CASP12 (qRT-PCR)
Caspase-dependent apoptosis pathway activation

Limitations

In vitro study in a cancer cell line (HT-29), not normal human colon epithelium
Specific exposure metric (e.g., SAR) not reported in the abstract
Sample size and replication details not provided in the abstract
Statistical reporting is incomplete/unclear in the abstract (e.g., p-value formatting)

View raw extracted JSON

{
  "study_type": "in_vitro",
  "exposure": {
    "band": "RF",
    "source": null,
    "frequency_mhz": null,
    "sar_wkg": null,
    "duration": "3 h continuous; 6 h intermittent; 6 h continuous"
  },
  "population": "Human colon adenocarcinoma (HT-29) cells",
  "sample_size": null,
  "outcomes": [
    "Cell viability (Trypan Blue exclusion assay)",
    "Gene expression of CASP8, CASP9, CASP3, CASP12 (qRT-PCR)",
    "Caspase-dependent apoptosis pathway activation"
  ],
  "main_findings": "HT-29 cells exposed to 1800, 2100, and 2600 MHz radiofrequency radiation showed frequency- and duration-dependent changes. Exposure at 2100 MHz for all three exposure durations was reported as more effective on the ratio of viable cells compared with 1800 and 2600 MHz, and 2100 MHz exposure was associated with significant increases in caspase activation (CASP8/CASP9 for 3 h continuous and 6 h intermittent; CASP3 for 6 h continuous).",
  "effect_direction": "harm",
  "limitations": [
    "In vitro study in a cancer cell line (HT-29), not normal human colon epithelium",
    "Specific exposure metric (e.g., SAR) not reported in the abstract",
    "Sample size and replication details not provided in the abstract",
    "Statistical reporting is incomplete/unclear in the abstract (e.g., p-value formatting)"
  ],
  "evidence_strength": "low",
  "confidence": 0.74,
  "peer_reviewed_likely": "yes",
  "keywords": [
    "radiofrequency radiation",
    "RFR",
    "HT-29",
    "colon adenocarcinoma",
    "cell viability",
    "apoptosis",
    "caspase",
    "CASP8",
    "CASP9",
    "CASP3",
    "CASP12",
    "1800 MHz",
    "2100 MHz",
    "2600 MHz"
  ],
  "suggested_hubs": []
}

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AI-extracted fields are generated from the abstract and metadata and may be incomplete or incorrect. This material is for informational purposes and is not medical advice.

Identifiers and source

DOI: 10.4149/bll_2019_050
PubMed: Not supplied

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